Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Pentanedione | pH profile for the inactivation, overview | Rattus norvegicus | |
3-Aminopyridine adenine dinucleotide phosphate | diazotized, coenzyme-competitive at 25°C, pH 7.4, reversible binding | Rattus norvegicus | |
Fluorescein mercuric acetate | - |
Rattus norvegicus | |
Maleimide | pH profile for the inactivation, overview | Rattus norvegicus | |
additional information | apparent second-order rate constants for inactivation are observed to increase with increasing chain length of the N-alkylmaleimide used, NADP-and coenzyme-competitive inhibitors such as 3-aminopyridine adenine dinucleotide phosphate and various adenosine derivatives protect the enzyme against maleimide inactivation, whereas no protection is observed with the steroid substrate, overview; NADP+, 3-aminopyridine adenine dinucleotide phosphate and three adenosine derivatives protect enzyme against inactivation by N-ethylmaleimide, 20alpha-hydroxyprogesterone does not and N1-ethylnicotinamide chloride not significantly; sulfhydryl groups are modified during inactivation | Rattus norvegicus | |
N-Alkylmaleimide | N-ethylmaleimide and N-butylmaleimide to N-octylmaleimide at 25°C, pH 7.7 | Rattus norvegicus | |
N-Butylmaleimide | inactivation via sulfhydryl groups of the enzyme in a nonpolar region | Rattus norvegicus | |
N-ethylmaleimide | inactivation via sulfhydryl groups of the enzyme in a nonpolar region | Rattus norvegicus | |
N-Heptylmaleimide | inactivation via sulfhydryl groups of the enzyme in a nonpolar region | Rattus norvegicus | |
N-Hexylmaleimide | inactivation via sulfhydryl groups of the enzyme in a nonpolar region | Rattus norvegicus | |
N-Octylmaleimide | inactivation via sulfhydryl groups of the enzyme in a nonpolar region | Rattus norvegicus | |
N-Pentylmaleimide | inactivation via sulfhydryl groups of the enzyme in a nonpolar region | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NAD(P)H | Rattus norvegicus | - |
20alpha-hydroxypregn-4-en-3-one + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20alpha-hydroxyprogesterone + NADP+ | - |
Rattus norvegicus | progesterone + NADPH | - |
? | |
progesterone + NAD(P)H | - |
Rattus norvegicus | 20alpha-hydroxypregn-4-en-3-one + NAD(P)+ | - |
r | |
progesterone + NAD(P)H | i.e. pregn-4-en-3-one, importance of sulfhydryl groups in the functioning of the enzyme | Rattus norvegicus | 20alpha-hydroxypregn-4-en-3-one + NAD(P)+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
20alpha-HSD | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Rattus norvegicus | |
NAD(P)H | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus |