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Literature summary for 1.1.1.136 extracted from
- Vi antigen biosynthesis in Salmonella typhi: characterization of UDP-N-acetylglucosamine C-6 dehydrogenase (TviB) and UDP-N-acetylglucosaminuronic acid C-4 epimerase (TviC) (2006), Biochemistry, 45, 8163-8173.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tviB located in the viaB locus, genetic organization, expression as C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.077 | - |
UDP-N-acetyl-D-glucosamine | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhi |  |
| 0.276 | - |
NAD+ | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Salmonella enterica subsp. enterica serovar Typhi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47675 | - |
3 * 47675, subunit amino acid sequence calculation | Salmonella enterica subsp. enterica serovar Typhi |
| 120500 | - |
recombinant enzyme, gel filtration | Salmonella enterica subsp. enterica serovar Typhi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + NAD+ + H2O | Salmonella enterica subsp. enterica serovar Typhi | the enzyme is involved in the biosynthesis of the Vi antigen, a clinically important vaccine antigen, in Salmonella typhi, pathway overview | UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhi | - |
ATCC 700931, gene tviB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged TviB from Escherichia coli strain BL21(DE3) by two steps of anion exchange chromatography, affinity chromatography, and gel filtration, inactive oligomeric forms of the enzym eis removed in gel filtration, to near homogeneity | Salmonella enterica subsp. enterica serovar Typhi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, purified recombinant enzyme, in presence of 10 mM DTT, stable, even to multiple freeze-thaw cycles | Salmonella enterica subsp. enterica serovar Typhi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | determination of substrate specificity, no or poor activity with UDP-D-glucose, UDP-D-galactose, and UDP-D-galactosamine, overview | Salmonella enterica subsp. enterica serovar Typhi | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + NAD+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhi | UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH | product determination by NMR | ? | |
| UDP-N-acetyl-D-glucosamine + NAD+ + H2O | the enzyme is involved in the biosynthesis of the Vi antigen, a clinically important vaccine antigen, in Salmonella typhi, pathway overview | Salmonella enterica subsp. enterica serovar Typhi | UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 47675, subunit amino acid sequence calculation | Salmonella enterica subsp. enterica serovar Typhi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TviB | - |
Salmonella enterica subsp. enterica serovar Typhi |
| UDP-N-acetylglucosamine C-6 dehydrogenase | - |
Salmonella enterica subsp. enterica serovar Typhi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | assay at | Salmonella enterica subsp. enterica serovar Typhi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15.5 | - |
NAD+ | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhi | |
| 15.5 | - |
UDP-N-acetyl-D-glucosamine | pH 7.5, 25°C, recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Salmonella enterica subsp. enterica serovar Typhi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.4 | inactive above pH 8.4 | Salmonella enterica subsp. enterica serovar Typhi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhi | |
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