Application | Comment | Organism |
---|---|---|
industry | Optimizing whole-cell biocatalysts by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucose facilitator from Zymomonas mobilis for uptake of unphosphorylated glucose and a NADP+-dependent glucose dehydrogenase from Bacillus megaterium that oxidizes glucose to gluconolactone. | Priestia megaterium |
synthesis | Escherichia coli strain expressing both recombinant glucose 1-dehydrogenase and a glucose facilitator for uptake of unphosphorylated glucose shows a nine times higher initial alpha-pinene oxide formation rate corresponding to a sixfold higher yield of 20 mg per g cell dry weight after 1.5 h and to a sevenfold increased alpha-pinene oxide yield in the presence of glucose compared to glucose-free conditions | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) already heterologously overexpressing P450 BM-3 QM together with glucose facilitator (GLF) from Zymomonas mobilis | Priestia megaterium |
expression in Escherichia coli, together with a glucose facilitator from Zymomonas mobilis for uptake of unphosphorylated glucose | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Escherichia coli strain expressing both recombinant glucose 1-dehydrogenase and a glucose facilitator for uptake of unphosphorylated glucose shows a nine times higher initial alpha-pinene oxide formation rate corresponding to a sixfold higher yield of 20 mg per g cell dry weight after 1.5 h and to a sevenfold increased alpha-pinene oxide yield in the presence of glucose compared to glucose-free conditions | Priestia megaterium |
additional information | introduction of both, GLF and GlcDH, in P450-overexpressing Escherichia coli should enable the cell to carry out a straightforward intracellular cofactor regeneration driven by externally added glucose. For the generation of recombinant Escherichia coli strains carrying two plasmids, these are transformed successively. Firstly, pZY507glf is transformed into Escherichia coli BL21 (DE3). Subsequently, competent cells are prepared from a positive transformant, and then pETDUETbm-3qm glcdh is transformed. | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NADP+ | Priestia megaterium | unphosphorylated glucose as substrate | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium | - |
expression in Escherichia coli BL21 (DE3) already heterologously overexpressing P450 BM-3 QM together with glucose facilitator (GLF) from Zymomonas mobilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NADP+ | unphosphorylated glucose as substrate | Priestia megaterium | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcDH 2 | - |
Priestia megaterium |
glucose dehydrogenase | - |
Priestia megaterium |