Cyclase I of Salvia officinalis (sage) gives about equal parts (+)-camphene and (+)-alpha-pinene. (3R)-Linalyl diphosphate can also be used by the enzyme in preference to (3S)-linalyl diphosphate. Requires Mg2+ (preferred to Mn2+). See also EC 4.2.3.121 (+)-alpha-pinene synthase.
Cyclase I of Salvia officinalis (sage) gives about equal parts (+)-camphene and (+)-alpha-pinene. (3R)-Linalyl diphosphate can also be used by the enzyme in preference to (3S)-linalyl diphosphate. Requires Mg2+ (preferred to Mn2+). See also EC 4.2.3.121 (+)-alpha-pinene synthase.
products are 25% (+)-alpha-pinene, 25% (+)-camphene, 43% (+)-limonene, and 7% myrcene. Reaction in organic solvent gives the same olefin distribution as in aqueous buffer
GC-MS analysis and identification of products from enzyme catalysis, overview. Enzyme AaTPS5 catalyzes the production of five monoterpenes, of which camphene is the major one which accounts for 52.12% of the total, and (-)-alpha-pinene (30.08%), (-)-beta-pinene (3.89%), tricyclene (2.68%), and beta-myrcene (1.23%) are the less abundant products
GC-MS analysis and identification of products from enzyme catalysis, overview. Enzyme AaTPS5 catalyzes the production of five monoterpenes, of which camphene is the major one which accounts for 52.12% of the total, and (-)-alpha-pinene (30.08%), (-)-beta-pinene (3.89%), tricyclene (2.68%), and beta-myrcene (1.23%) are the less abundant products
enzyme removes the C4-proR-hydrogen of the substrate, the C3 proton trans to the dimethyl bridge of the pinyl nucleus, with a stereoselectivity exceeding 94% in the formation of (+)-alpha-pinene
product distribution varies with deuterium substitution at C4 and C10 of substrate. Kinetic isotope effects strongly indicate multiple bicyclic olefin production through the partitioning of common carbocation intermediates
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Whooping Cough
Interactions of Bordetella pertussis adenylyl cyclase toxin CyaA with calmodulin mutants and calmodulin antagonists: Comparison with membranous adenylyl cyclase I.
the N-terminal signal peptide of AaTPS5 (57 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3)
the N-terminal signal peptide of AaTPS5 (57 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3)
enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene
enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene
enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene. Reactions run in hexane generate product at roughly one-third the rate of companion assays in which the lyophilized protein is rehydrated in distilled water
enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AaTPS5, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme, lacking the N-terminal signal peptide, in Escherichia coli strain BL21(DE3)
Biosynthesis of monoterpenes: Enantioselectivity in the enzymatic cyclization of (+)- and (-)-linalyl pyrophospahte to (+)- and (-) pinene and (+)- and (-)-camphene
Pinene cyclases I and II. Two enzymes from sage (Salvia officinalis) which catalyze stereospecific cyclizations of geranyl pyrophosphate to monoterpene olefins of opposite configuration