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EC Tree
The expected taxonomic range for this enzyme is: Faecalicatena orotica
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N-carbamoyl-L-aspartate + H2O = L-aspartate + CO2 + NH3
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hydrolysis of peptide bond
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N-carbamoyl-L-aspartate amidohydrolase
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carbamoylglutamate + H2O
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small amount of activity found
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L-carboxymethylhydantoin + H2O
CO2 + ?
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inert substrate
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L-ureidosuccinic acid + H2O
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only the L-isomer is attacked, the D form is completely inactive
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L-ureidosuccinic acid + H2O
L-aspartic acid + CO2 + NH3
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only the L-isomer was attacked, the D form is completely inactive
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additional information
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L-carboxymethylhydantoin is no substrate
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L-ureidosuccinic acid + H2O
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only the L-isomer is attacked, the D form is completely inactive
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additional information
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L-carboxymethylhydantoin is no substrate
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?
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Mn2+
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absolute requirement
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glutathione
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effect less than half that of cysteine
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brenda
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0°C stable for at least 10 days if kept in vacuo, loses activity rapidly when exposed to air either at 0°C or -16°C
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Lieberman, I.; Kornberg, A.
Enzymatic synthesis and breakdown of a pyrimidine, orotic acid. III. ureidosuccinase
J. Biol. Chem.
212
909-920
1955
Faecalicatena orotica
brenda
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