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EC Tree
The expected taxonomic range for this enzyme is: Hypericum
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aromatic prenyltransferase
apT
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aromatic prenyltransferase
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aromatic prenyltransferase
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PT8px
isoform
PTpat
isoform
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dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone = diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
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dimethylallyl diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
diphosphate + patulone
dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone
diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
dimethylallyl diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
diphosphate + patulone
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-
-
?
dimethylallyl diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
diphosphate + patulone
100% activity with isoform PTpat
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-
?
dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone
diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
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?
dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone
diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
100% activity with isoform PT8px
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-
?
dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone
diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone
4.6% activity with isoform PTpat
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-
?
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Mg2+
6 mM used in assay conditions
Mg2+
6 mM used in assay conditions
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Tuberculosis
The Influence of Mycobacterium tuberculosis sigma factors on the promotion efficiency of ptpAt promoter in Mycobacterium smegmatis.
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0.0736 - 0.0838
1,3,6,7-tetrahydroxy-8-prenylxanthone
0.0532 - 0.2
1,3,6,7-tetrahydroxyxanthone
0.0158 - 0.0237
dimethylallyl diphosphate
0.0736
1,3,6,7-tetrahydroxy-8-prenylxanthone
isoform PTpat, at pH 7.5 and 37°C
0.0838
1,3,6,7-tetrahydroxy-8-prenylxanthone
isoform PT8px, at pH 7.0 and 37°C
0.0532
1,3,6,7-tetrahydroxyxanthone
isoform PT8px, at pH 7.0 and 37°C
0.2
1,3,6,7-tetrahydroxyxanthone
isoform PTpat, at pH 7.5 and 37°C
0.0158
dimethylallyl diphosphate
isoform PT8px, at pH 7.0 and 37°C
0.0237
dimethylallyl diphosphate
isoform PTpat, at pH 7.5 and 37°C
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7 - 7.5
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35 - 40
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UniProt
brenda
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UniProt
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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A0A3Q8S421_9ROSI
380
0
41928
TrEMBL
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A0A3S5HJL9_9ROSI
392
0
42903
TrEMBL
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A0A3S8RK07_9ROSI
386
0
43185
TrEMBL
Secretory Pathway (Reliability: 2)
A0A451ENJ9_9ROSI
380
0
42140
TrEMBL
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expressed in Nicotiana benthamiana
expressed in Saccharomyces cerevisiae strain InvSc1
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Nagia, M.; Gaid, M.; Biedermann, E.; Fiesel, T.; El-Awaad, I.; Haensch, R.; Wittstock, U.; Beerhues, L.
Sequential regiospecific gem-diprenylation of tetrahydroxyxanthone by prenyltransferases from Hypericum sp.
New Phytol.
222
318-334
2019
Hypericum calycinum (A0A3Q8S421), Hypericum calycinum (A0A3S8RK07), Hypericum sampsonii (A0A3S5HJL9), Hypericum sampsonii (A0A451ENJ9)
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