We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
beta-gt, gt-b glycosyltransferase, t4 beta-glucosyltransferase, t4 phage beta-glucosyltransferase, dna beta-glucosyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
beta-GT
Tequatrovirus T4
-
-
glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate beta-
-
-
-
-
GT-B glycosyltransferase
Tequatrovirus T4
-
-
T4 beta-glucosyl transferase
Tequatrovirus T4
-
-
T4 beta-glucosyltransferase
Tequatrovirus T4
-
-
T4 phage beta-glucosyltransferase
Tequatrovirus T4
-
-
T4-beta-glucosyl transferase
Tequatrovirus T4
-
-
T4-HMC-beta-glucosyl transferase
Tequatrovirus T4
-
-
UDP glucose-DNA beta-glucosyltransferase
-
-
-
-
uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase
-
-
-
-
additional information
Tequatrovirus T4
-
the enzyme belongs to the GT-B glycosyltransferase family
BGT
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
catalytic mechanism
Tequatrovirus T4
-
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
base-flipping mechanism
Tequatrovirus T4
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
catalytic mechanism, substrate binding structures, overview
Tequatrovirus T4
-
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hexosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-glucose:DNA beta-D-glucosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-alpha-D-glucose + 5-hydroxymethylcytosine-containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine-containing DNA
Tequatrovirus T4
-
-
-
-
-
UDP-D-glucose + 5-hydroxymethyl cytosine
UDP + 5-hydroxymethyl cytosine beta-D-glucoside
Tequatrovirus T4
-
5-hydroxymethyl cytosine residues of duplex DNA
-
-
?
UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
-
recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme
-
?
UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA
Tequatrovirus T4
-
-
-
-
?
UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
-
-
-
-
?
UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
-
-
-
-
?
UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
-
-
-
-
?
UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
-
-
-
-
?
additional information
?
-
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
-
-
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
DNA-modifying enzyme
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme forms part of a phage DNA protection system
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
the enzyme modifies T4-DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview
-
-
?
additional information
?
-
Tequatrovirus T4
-
the alpha-linkage forming enzyme AGT is also able to adopt the fold of a GT-B glycosyltransferase keeping the sugar donor binding mechanism typical for GT-B glycosyltransferases, but showing a unique sugar acceptor binding mode, both substrates are bound to the C-terminal domain, detailed substrate binding structures, catalytic mechanism of both enzyme types, overview
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-alpha-D-glucose + 5-hydroxymethylcytosine-containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine-containing DNA
Tequatrovirus T4
-
-
-
-
-
UDP-D-glucose + 5-hydroxymethyl cytosine
UDP + 5-hydroxymethyl cytosine beta-D-glucoside
Tequatrovirus T4
-
5-hydroxymethyl cytosine residues of duplex DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
-
-
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
DNA-modifying enzyme
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme forms part of a phage DNA protection system
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
the enzyme modifies T4-DNA
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
Tequatrovirus T4
-
four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Mg2+
Tequatrovirus T4
-
four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Mn2+
Tequatrovirus T4
-
four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-phenylimidazole
Tequatrovirus T4
-
-
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
competitive inhibitor with respect to 5-hydroxymethyl cytosine containing DNA and mixed inhibitor with respect to UDP-glucose
UDP
Tequatrovirus T4
-
competitive inhibitor with respect to UDP-glucose and mixed inhibitor with respect to 5-hydroxymethyl cytosine containing DNA
uridine 5-diphosphate
Tequatrovirus T4
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0004
5-hydroxymethyl cytosine containing phage T4 DNA
Tequatrovirus T4
-
pH 7.9, 25°C
-
0.00023
DNA containing 12 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
0.003
DNA containing 2 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
0.00022
DNA containing 24 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
0.0006
DNA containing 6 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
0.016
UDP-D-glucose
Tequatrovirus T4
-
pH 7.9, 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.283
5-hydroxymethyl cytosine containing phage T4 DNA
Tequatrovirus T4
-
pH 7.9, 25°C
-
1.033
DNA containing 12 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
2.8
DNA containing 2 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
9.66
DNA containing 24 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
1.666
DNA containing 6 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3200
5-hydroxymethyl cytosine containing phage T4 DNA
Tequatrovirus T4
-
pH 7.9, 25°C
-
4483
DNA containing 12 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
933
DNA containing 2 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
4400
DNA containing 24 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
2766
DNA containing 6 5-hydroxymethyl cytosine residues
Tequatrovirus T4
-
pH 7.9, 25°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.003 - 0.004
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
0.003
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
substrate UDP-glucose, pH 7.9, 25°C
0.004
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C
0.002
UDP
Tequatrovirus T4
-
substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C
0.009
UDP
Tequatrovirus T4
-
substrate UDP-glucose, pH 7.9, 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00214 - 0.00422
4-phenylimidazole
0.01354
uridine 5-diphosphate
Tequatrovirus T4
-
at pH 7.4 and 37°C
0.00214
4-phenylimidazole
Tequatrovirus T4
-
at pH 7.4 and 37°C
0.00422
4-phenylimidazole
Tequatrovirus T4
-
at pH 7.4 and 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tequatrovirus T4
-
-
-
brenda
Tequatrovirus T4
-
Uniprot
brenda
Tequatrovirus T4
-
-
-
brenda
Tequatrovirus T4
-
Uniprot
brenda
Tequatrovirus T4
-
-
-
brenda
Tequatrovirus T4
-
Uniprot
brenda
Tequatrovirus T4
-
-
-
brenda
Tequatrovirus T4
from Escherichia coli
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tequatrovirus T4
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GSTB_BPT4
351
0
40666
Swiss-Prot
other Location (Reliability: 2 )
A0A8K1S473_9CAUD
358
0
41781
TrEMBL
other Location (Reliability: 2 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
Tequatrovirus T4
-
structural analysis of domain folding, substrate binding, catalytic mechanism, and base flipping mechanism, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
crystal structure of the recombinant enzyme in the presence and absence of the substrate, UDPglucose
Tequatrovirus T4
-
crystallization of the enzyme with UDPglucose and a 13mer DNA fragment containing an abasic site. Two crystal structures are obtained of a ternary complex BGT-UDP-DNA at 1.8 A and 2.5 A resolution, one with a Tris molecule and the other with a metal ion at the active site
Tequatrovirus T4
ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
Tequatrovirus T4
-
ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
Tequatrovirus T4
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
Tequatrovirus T4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
analysis
Tequatrovirus T4
-
use of recombinant enzyme to estimate global 5-hydroxymethyl cytosine content in a variety of genomic DNAs. Most of the genomic DNAs derived from vertebrate tissue and cell lines contain 5-hydroxymethyl cytosine. DNA from mouse, human, and bovine brains displays 0.5?0.9% of the total nucleotides as 5-hydroxymethyl cytosine, which is higher compared to the levels found in other tissues. A comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer
medicine
Tequatrovirus T4
-
a comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Freemont, P.S.; Ruger, W.
Crystallization and preliminary X-ray studies of T4 phage beta-glucosyltransferase
J. Mol. Biol.
203
525-526
1988
Tequatrovirus T4
brenda
Lariviere, L.; Kurzeck, J.; Aschke-Sonnenborn, U.; Rueger, W.; Morera, S.
Crystallization and preliminary crystallographic study of a ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
Acta Crystallogr. Sect. D
58
1484-1486
2002
Tequatrovirus T4 (P04547)
brenda
Morera, S.; Lariviere, L.; Kurzeck, J.; Aschke-Sonnenborn, U.; Freemont, P.S.; Janin, J.; Ruger, W.
High resolution crystal structures of T4 phage beta-glucosyltransferase: Induced fit and effect of substrate and metal binding
J. Mol. Biol.
311
569-577
2001
Tequatrovirus T4
brenda
Vrielink, A.; Rueger, W.; Driessen, H.P.C.; Freemont, P.S.
Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose
EMBO J.
13
3413-3422
1994
Tequatrovirus T4
brenda
Morera, S.; Imberty, A.; Aschke-Sonnenborn, U.; Ruger, W.; Freemont, P.S.
T4 phage beta-glucosyltransferase: Substrate binding and proposed catalytic mechanism
J. Mol. Biol.
292
717-730
1999
Tequatrovirus T4
brenda
Lariviere, L.; Morera, S.
A base-flipping mechanism for the T4 phage beta-glucosyltransferase and identification of a transition-state analog
J. Mol. Biol.
324
483-490
2002
Tequatrovirus T4 (P04547)
brenda
Artymiuk, P.J.; Rice, D.W.; Poirrette, A.R.; Willett, P.
beta-Glucosyltransferase and phosphorylase reveal their common theme
Nat. Struct. Biol.
2
117-120
1995
Tequatrovirus T4
brenda
Lariviere, L.; Gueguen-Chaignon, V.; Morera, S.:
Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: Glucose binding and identification of the catalytic base for a direct displacement mechanism
J. Mol. Biol.
330
1077-1086
2003
Tequatrovirus T4 (P04547)
brenda
Lariviere, L.; Sommer, N.; Morera, S.
Structural evidence of a passive base-flipping mechanism for AGT, an unusual GT-B glycosyltransferase
J. Mol. Biol.
352
139-150
2005
Tequatrovirus T4
brenda
Terragni, J.; Bitinaite, J.; Zheng, Y.; Pradhan, S.
Biochemical characterization of recombinant alpha-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes
Biochemistry
51
1009-1019
2012
Tequatrovirus T4
brenda
Yin, H.; Yang, Z.; Wang, H.; Zhou, Y.; Ai, S.
Electrochemical biosensor for hydroxymethylated DNA detection and beta-glucosyltransferase activity assay based on enzymatic catalysis triggering signal amplification
Sens. Actuators B Chem.
243
602-608
2017
Tequatrovirus T4
-
brenda
Jiang, W.; Yin, H.; Zhou, Y.; Duan, J.; Li, H.; Wang, M.; Waterhouse, G.; Ai, S.
A novel electrochemiluminescence biosensor for the detection of 5-methylcytosine, TET 1 protein and beta-glucosyltransferase activities based on gold nanoclusters-H2O2 system
Sens. Actuators B Chem.
274
144-151
2018
Tequatrovirus T4
-
brenda
Select items on the left to see more content.
html completed