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EC Tree
IUBMB Comments The enzyme participates in the biosynthesis of luteolin triglucuronide, the major flavone found in the photosynthetically-active mesophyll of the primary leaves of Secale cereale (rye).
The expected taxonomic range for this enzyme is: Secale cereale
Synonyms
glucuronosyltransferase, uridine diphosphoglucuronate-luteolin 7-O-glucuronide, LMT, luteolin-7-O-glucuronide 7-O-glucuronosyltransferase, UDP-glucuronate:luteolin 7-O-glucuronide-glucuronosyltransferase, UDP-glucuronate:luteolin-7-O-beta-D-glucuronide 7-O-glucuronosyltransferase, UDPglucuronate:luteolin-7-O-beta-D-glucuronide 7-O-glucuronosyltransferase,
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glucuronosyltransferase, uridine diphosphoglucuronate-luteolin 7-O-glucuronide
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luteolin-7-O-glucuronide 7-O-glucuronosyltransferase
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UDP-glucuronate:luteolin 7-O-glucuronide-glucuronosyltransferase
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UDP-glucuronate:luteolin-7-O-beta-D-glucuronide 7-O-glucuronosyltransferase
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UDPglucuronate:luteolin-7-O-beta-D-glucuronide 7-O-glucuronosyltransferase
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UDP-alpha-D-glucuronate + luteolin 7-O-beta-D-glucuronide = UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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hexosyl group transfer
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UDP-glucuronate:luteolin-7-O-beta-D-glucuronide 2''-O-glucuronosyltransferase
The enzyme participates in the biosynthesis of luteolin triglucuronide, the major flavone found in the photosynthetically-active mesophyll of the primary leaves of Secale cereale (rye).
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UDP-glucuronic acid + apigenin 7-O-beta-D-glucuronide
UDP + apigenin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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88% of activity with luteolin 7-O-glucuronide
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UDP-glucuronic acid + chrysoeriol 7-O-beta-D-glucuronide
UDP + chrysoeriol 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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65% of activity with luteolin-7-O-glucuronide
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UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDPglucuronic acid + apigenin 7-O-glucoside
UDP + apigenin-7-O-beta-D-glucosyl-1,4-beta-D-glucuronoside
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51.5% of activity with luteolin 7-O-glucuronide
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UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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ir
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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ir
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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involved in the sequential biosynthesis of luteolin triglucuronide
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UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
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involved in the sequential biosynthesis of luteolin triglucuronide
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Ag+
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0.75 mM, 40% inhibition
Cu2+
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0.75 mM, 40% inhibition
luteolin
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0.1 mM, 50% inhibition
Pb2+
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0.75 mM, 40% inhibition
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0.012
luteolin 7-O-glucuronide
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0.04
UDPglucuronic acid
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8.5
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pH-optimum at pH 8.5 is absent in crude enzyme preparations
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6 - 9
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50% of maximal activity at pH 6.0 and pH 9.0
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rye
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brenda
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brenda
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no activity in vacuoles
brenda
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freezing without glycerol, complete inactivation
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-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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Ultrogel AcA 44, ultrafiltration, hydroxylapatite, partial purification
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Schulz, M.; Weissenbck, G.
Three specific UDP-glucuronate: flavone-glucuronosyl-transferases from primary leaves of Secale cereale
Phytochemistry
27
1261-1267
1988
Secale cereale
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brenda
Anhalt, S.; Weissenbck, G.
Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll
Planta
187
83-88
1992
Secale cereale
brenda
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