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IUBMB CommentsCoumarinate (cis-2-hydroxycinnamate) does not act as acceptor.
The expected taxonomic range for this enzyme is: Melilotus albus
Synonyms
glucosyltransferase, uridine diphosphoglucose-o-coumarate, UDPG:o-coumaric acid O-glucosyltransferase,
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glucosyltransferase, uridine diphosphoglucose-o-coumarate
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UDPG:o-coumaric acid O-glucosyltransferase
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UDP-glucose + trans-2-hydroxycinnamate = UDP + trans-beta-D-glucosyl-2-hydroxycinnamate
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hexosyl group transfer
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UDP-glucose:trans-2-hydroxycinnamate O-beta-D-glucosyltransferase
Coumarinate (cis-2-hydroxycinnamate) does not act as acceptor.
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UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
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UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
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UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
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involved in the biosynthesis of coumarin
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?
UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
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involved in the biosynthesis of coumarin
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?
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UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
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involved in the biosynthesis of coumarin
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?
UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
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involved in the biosynthesis of coumarin
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?
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Cu2+
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1 mM, 63% inhibition
Sodium citrate
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4 mM, 37% inhibition
Zn2+
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1 mM, 77% inhibition
additional information
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not inhibitory: 4 mM EDTA or 0.8 mM 2-coumaric acid glucoside
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2-mercaptoethanol
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stimulation
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6.5 - 8.8
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25% of maximal activity at pH 6, 10% of maximal activity at pH 9
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cucubb genotype
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brenda
var. White Blossom
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brenda
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brenda
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additional information
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not associated with chloroplasts
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brenda
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4
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1-2 d, complete loss of activity
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dialysis overnight inactivates
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freezing results in a complete loss of activity
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4°C, complete loss of activity within 1-2 days
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Kleinhofs, A.; Haskins, F.A.; Gorz, H.J.
trans-o-Hydroxycinnamic acid glucosylation in cell-free extracts of Meliotus alba
Phytochemistry
6
1313-1318
1967
Melilotus albus
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brenda
Poulton, J.E.; McRee, D.E.; Conn, E.E.
Intracellular localization of two enzymes involved in coumarin biosynthesis in Meliotus alba
Plant Physiol.
65
171-175
1980
Melilotus albus
brenda
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2.4.1.114 2-coumarate O-beta-glucosyltransferase
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