Galactarate can act as acceptor, more slowly. Involved with EC 2.3.1.99 quinate O-hydroxycinnamoyltransferase in the formation of caffeoylglucarate in tomato.
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The enzyme appears in viruses and cellular organisms
Galactarate can act as acceptor, more slowly. Involved with EC 2.3.1.99 quinate O-hydroxycinnamoyltransferase in the formation of caffeoylglucarate in tomato.
treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively
tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers
tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers
the sequence determines SlCGT as a GDSL lipase-like protein, representing it as a member of the SGNH protein superfamily. Lipases of this family employ a catalytic triad of Ser-Asp-His with Ser as nucleophile of the GDSL motif. The catalytic triad of the GDSL lipase is not essential for SlCGT enzymatic activity. SlCGT is therefore an example of a GDSL lipase-like protein that has lost hydrolytic activity and has acquired a completely new function in plant metabolism, functioning in secondary metabolism as acyltransferase in synthesis of hydroxycinnamate esters by employing amino acid residues different from the lipase catalytic triad
the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT
the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
native SlCGT 585fold from seedlings by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography