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EC Tree
The expected taxonomic range for this enzyme is: Quercus rubra
Synonyms
beta-glucogallin:1,2,3,6-tetra-O-galloyl-beta-D-glucose 4-O-galloyltransferase, beta-glucogallin:1,2,3,6-tetra-O-galloylglucose 4-O-galloyltransferase, galloyltransferase, beta-glucogallin-tetragalloylglucose 4-, tetragalloylglucose 4-O-galloyltransferase,
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beta-glucogallin:1,2,3,6-tetra-O-galloyl-beta-D-glucose 4-O-galloyltransferase
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beta-glucogallin:1,2,3,6-tetra-O-galloylglucose 4-O-galloyltransferase
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galloyltransferase, beta-glucogallin-tetragalloylglucose 4-
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tetragalloylglucose 4-O-galloyltransferase
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1-O-galloyl-beta-D-glucose + 1,2,3,6-tetrakis-O-galloyl-beta-D-glucose = D-glucose + 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose
1-O-galloyl-beta-D-glucose + 1,2,3,6-tetrakis-O-galloyl-beta-D-glucose = D-glucose + 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose
stoichiometry
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1-O-galloyl-beta-D-glucose + 1,2,3,6-tetrakis-O-galloyl-beta-D-glucose = D-glucose + 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose
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Acyl group transfer
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1-O-galloyl-beta-D-glucose:1,2,3,6-tetrakis-O-galloyl-beta-D-glucose 4-O-galloyltransferase
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1-O-galloyl-beta-D-glucose + 1,2,3,6-tetra-O-galloyl-beta-D-glucose
D-glucose + 1,2,3,4,6-penta-O-galloyl-beta-D-glucose
additional information
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the enzyme catalyzes the last common step in the biosynthesis of hydrolyzable tannins
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1-O-galloyl-beta-D-glucose + 1,2,3,6-tetra-O-galloyl-beta-D-glucose
D-glucose + 1,2,3,4,6-penta-O-galloyl-beta-D-glucose
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1-O-galloyl-beta-D-glucose + 1,2,3,6-tetra-O-galloyl-beta-D-glucose
D-glucose + 1,2,3,4,6-penta-O-galloyl-beta-D-glucose
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1-O-galloyl-beta-D-glucose is beta-glucogallin, no activity with 1,2,4,6-isomer
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additional information
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the enzyme catalyzes the last common step in the biosynthesis of hydrolyzable tannins
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1
1,2,3,6-tetra-O-galloyl-beta-D-glucose
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2.3
1-O-Galloyl-beta-D-glucose
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additional information
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4 - 8
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50% of maximal activity at pH 4.0 and pH 8.0
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additional information
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some activity at 0°C
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oak
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brenda
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brenda
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65000
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4 * 65000, SDS-PAGE
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homotetramer
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4 * 65000, SDS-PAGE
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0-4°C, within 1-2 weeks no significant loss of activity
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O2-sensitive, buffers have to be supplemented with 5 mM 2-mercaptoethanol
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486327
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Cammann, J.; Denzel, K.; Schilling, G.; Gross, G.
Biosynthesis of gallotannins: beta-glucogallin-dependent formation of 1,2,3,4,6-pentagalloylglucose by enzymatic galloylation of 1,2,3,6-tetragalloylglucose
Arch. Biochem. Biophys.
273
58-63
1989
Quercus rubra
brenda
Grundhofer, P.; Gross, G.G.
Purification of tetragalloylglucose 4-O-galloyltransferase and preparation of antibodies against this key enzyme in the biosynthesis of hydrolyzable tannins
Z. Naturforsch. C
55
582-587
2000
Quercus rubra
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brenda
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