We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme from the rice bean Vigna umbellata (Fabaceae) is highly specific for S-adenosyl-L-methionine. The enzyme also methylates 1L-1,2,4/3,5-cyclohexanepentol, 2,4,6/3,5-pentahydroxycyclohexanone, D,L-2,3,4,6/5-pentacyclohexanone and 2,2'-anhydro-2-C-hydroxymethyl-myo-inositol, but at lower rates than that of myo-inositol.
The enzyme appears in viruses and cellular organisms
Synonyms
imt, pcimt1, myo-inositol o-methyltransferase, inositol methyl transferase, myo-inositol 6-o-methyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC 2.1.1.134
-
-
formerly
-
inositol 6-O-methyltransferase
-
-
-
-
inositol methyl transferase
-
methyltransferase, inositol 6-O
-
-
-
-
methyltransferase, inositol L-1- (Mesembryanthemum crystallinum clone Imt1 reduced)
-
-
-
-
myo-inositol 4-O-methyltransferase
-
-
-
-
myo-inositol 6-O-methyltransferase
-
-
-
-
myo-inositol O-methyltransferase
S-adenosyl-L-methionine:myo-inositol 6-O-methyltransferase
-
-
-
-
IMT
-
myo-inositol O-methyltransferase
-
-
myo-inositol O-methyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + myo-inositol = S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
methyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine:1D-myo-inositol 4-methyltransferase
The enzyme from the rice bean Vigna umbellata (Fabaceae) is highly specific for S-adenosyl-L-methionine. The enzyme also methylates 1L-1,2,4/3,5-cyclohexanepentol, 2,4,6/3,5-pentahydroxycyclohexanone, D,L-2,3,4,6/5-pentacyclohexanone and 2,2'-anhydro-2-C-hydroxymethyl-myo-inositol, but at lower rates than that of myo-inositol.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
169277-48-9
formerly EC 2.1.1.134
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + 1L-1,3,4/2,5-cyclohexanepentol
?
-
i.e. L-viburnitol
-
-
?
S-adenosyl-L-methionine + 2,4,6/3,5-pentahydroxy-cyclohexanone
?
-
i.e. myo-inosose-2
-
-
?
S-adenosyl-L-methionine + D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
?
-
i.e. myo-inosose-4
-
-
?
S-adenosyl-L-methionine + myo-inositol
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 4-methyl-myo-inositol
-
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + D-ononitol
S-adenosyl-L-methionine + myo-inositol
?
-
the enzyme catalyzes the first step in the biosynthesis of the cyclic sugar alcohol pinitol. The presence of high levels of sugar alcohols correlates with osmotolerance
-
-
?
S-adenosyl-L-methionine + myo-inositol
?
-
the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
during normal growth IMT is repressed, after salinity stress IMT is induced. By inducing expression of IMT and increasing myo-inositol synthesis, metabolic end products accumulate, facilitating sodium sequestration and protecting photosynthesis
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
involved in the biosynthesis of pinitol, which is important for osmotic stress response
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
-
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
accumulation of methylated inositol in plants under salt-stress
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
-
1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stresses
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + D-ononitol
-
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + D-ononitol
-
-
D-ononitol is 1-D-4-O-methyl-myo-inositol
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + myo-inositol
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
S-adenosyl-L-methionine + myo-inositol
?
-
the enzyme catalyzes the first step in the biosynthesis of the cyclic sugar alcohol pinitol. The presence of high levels of sugar alcohols correlates with osmotolerance
-
-
?
S-adenosyl-L-methionine + myo-inositol
?
-
the product 1D-4-O-methyl-myo-inositol is accumulated in response to abiotic stress
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
during normal growth IMT is repressed, after salinity stress IMT is induced. By inducing expression of IMT and increasing myo-inositol synthesis, metabolic end products accumulate, facilitating sodium sequestration and protecting photosynthesis
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
involved in the biosynthesis of pinitol, which is important for osmotic stress response
-
-
?
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
K+
-
5 mM, slight stimulation
Na+
-
5 mM, slight stimulation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1L-1,3,4/2,5-cyclohexanepentol
-
i.e. L-viburnitol
2,2'-Anhydro-2-C-hydroxymethyl-myo-inositol
-
-
2,4,6/3,5-pentahydroxy-cyclohexanone
-
i.e. myo-inosose-2
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
-
i.e. myo-inosose-4
p-chloromercuribenzenesulfonic acid
-
0.02 mM
S-adenosyl-L-homocysteine
-
most potent inhibitor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dithiothreitol
-
1-20 mM, slight activation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.47
1-L-1,3,4/2,5-cyclohexanepentol
-
-
0.67
2,4,6/3,5-pentahydroxy-cyclohexanone
-
-
0.3
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
-
-
0.063
S-adenosyl-L-methionine
-
with myo-inositol as cosubstrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
proteome profile during salt-stress, up-regulation of RNA and protein expression following exposure to salinity, profiling of reaction product by gas chromatography, functional characterization of recombinant protein, regulation of pinitol synthesis pathway under different abiotic stresses, different plant species known to produce pinitol in response to stress listed, accumulation of D-pinitol via inositol is a stress-regulated pathway, presence of D-pinitol synthesizing protein/gene in a wild halophytic rice, absence in domesticated rice, adaptive feature in salt tolerance
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7 - 8.8
-
about 35% of maximal activity at pH 7.0 and pH 8.8
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30 - 55
-
30°C: about 40% of maximal activity, 55°C: about 30% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
no activity in Oryza sativa
-
-
-
brenda
Roxb. Tateoka
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
SwissProt
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
during normal growth IMT is repressed, after salinity stress IMT is induced
brenda
-
abiotic stress treatment
brenda
during normal growth IMT is repressed, after salinity stress IMT is induced
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
after cold stress, the Imt1 transgenic Arabidopsis plants exhibit stronger growth than the wild type plants
physiological function
tobacco plants transformed with the IMT gene produce up to 2.38 mg of D-ononitol per gram dry weight of leaves. Introduction of IMT, OEPA displaying NAD+-dependent D-ononitol dehydrogenase activity, and OEPB having NADP+-dependent D-pinitol dehydrogenase activity into tobacco plants results in production of D-ononitol and D-pinitol in transformants
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IMT1_MESCR
365
1
40296
Swiss-Prot
other Location (Reliability: 2 )
G7JTH4_MEDTR
369
0
41362
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
40000
-
x * 40000, SDS-PAGE
40250
-
x * 40250, calculation from nucleotide sequence
40300
-
2 * or 3 * 40300, SDS-PAGE
additional information
-
40 kDa immunoreactive protein under stressed and unstressed conditions in wild rice, an about 60 kDa major band detected by antibodies in domesticated and in wild rice
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 41360, calculated from sequence
?
-
x * 40250, calculation from nucleotide sequence
?
-
2 * or 3 * 40300, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.5
-
3 h, at room temperature, about 45% loss of activity compared to maximal stability at pH 7.0-7.6
288646
6.5
-
3 h, at room temperature, about 10% loss of activity compared to maximal stability at pH 7.0-7.6
288646
7 - 7.6
-
3 h, at room temperature, maximal stability
288646
8
-
3 h, at room temperature, about 30% loss of activity compared to maximal stability at pH 7.0-7.6
288646
9
-
3 h, at room temperature, about 70% loss of activity compared to maximal stability at pH 7.0-7.6
288646
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45
-
30 min, stable up to
50
-
30 min, about 40% loss of activity
60
-
30 min, complete loss of activity
70
-
15 min, sensitive to heat
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
activity is lost within a week, when the enzyme is frozen or kept at 4°C without glycerol
-
sensitive to proteinase K treatment
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-30°C, 25 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol, 2 mM MgCl2, 0.25 mM ammonium sulfate, 50% glycerol, half-life: 300 d
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gel filtration and SDS-PAGE, recombinant protein
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Arabidopsis thaliana cultivar Columbia
-
expressed in Escherichia coli BL21DE3 PLys(S) strain, pET15b vector
-
expression in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Vernon, D.M.; Bohnert, H.J.
A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum crystallinum
EMBO J.
11
2077-2085
1992
Mesembryanthemum crystallinum
brenda
Wanek, W.; Richter, A.
Purification and characterization of myo-inositol 6-O-methyltransferase from Vigna umbellata Ohwi et Ohashi
Planta
197
427-434
1995
Vigna umbellata
-
brenda
Nelson, D.E.; Rammesmayer, G.; Bohnert, H.J.
Regulation of cell-specific inositol metabolism and transport in plant salinity tolerance
Plant Cell
10
753-764
1998
Mesembryanthemum crystallinum (P45986)
brenda
Chiera, J.M.; Streeter, J.G.; Finer, J.J.
Ononitol and pinitol production in transgenic soybean containing the inositol methyl transferase gene from Mesembryanthemum crystallinum
Plant Sci.
171
647-654
2006
Mesembryanthemum crystallinum (P45986)
brenda
Sengupta, S.; Patra, B.; Ray, S.; Majumder, A.L.
Inositol methyl tranferase from a halophytic wild rice, Porteresia coarctata Roxb. (Tateoka): regulation of pinitol synthesis under abiotic stress
Plant Cell Environ.
31
1442-1459
2008
no activity in Oryza sativa, Oryza coarctata
brenda
Zhu, B.; Peng, R.; Xiong, A.; Xu, J.; Fu, X.; Zhao, W.; Jin, X.; Meng, X.; Gao, J.; Cai, R.; Yao, Q.
Transformation with a gene for myo-inositol O-methyltransferase enhances the cold tolerance of Arabidopsis thaliana
Biol. Plant.
56
135-139
2012
Mesembryanthemum crystallinum
-
brenda
Pupel, P.; Szablinska-Piernik, J.; Lahuta, L.B.
Two-step D-ononitol epimerization pathway in Medicago truncatula
Plant J.
100
237-250
2019
Medicago truncatula (G7JTH4), Medicago truncatula
brenda
Select items on the left to see more content.
html completed