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IUBMB Comments3,4-Dehydro-6-hydroxymellein can also act as acceptor. 6-Methoxymellein is a phytoalexin produced by carrot tissue.
The expected taxonomic range for this enzyme is: Daucus carota
Synonyms
6-hydroxymethyllein-methyltransferase, o-methyltransferase, 6-hydroxymellein, S-adenosyl-L-methionine:6-hydroxymellein O-methyltransferase,
more
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6-hydroxymethyllein-methyltransferase
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o-methyltransferase, 6-hydroxymellein
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S-adenosyl-L-methionine:6-hydroxymellein O-methyltransferase
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S-adenosyl-L-methionine + 6-hydroxymellein = S-adenosyl-L-homocysteine + 6-methoxymellein
S-adenosyl-L-methionine + 6-hydroxymellein = S-adenosyl-L-homocysteine + 6-methoxymellein
mechanism
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S-adenosyl-L-methionine + 6-hydroxymellein = S-adenosyl-L-homocysteine + 6-methoxymellein
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methyl group transfer
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S-adenosyl-L-methionine:6-hydroxymellein 6-O-methyltransferase
3,4-Dehydro-6-hydroxymellein can also act as acceptor. 6-Methoxymellein is a phytoalexin produced by carrot tissue.
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S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein
S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein
S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
S-adenosyl-L-methionine + 6-hydroxymellein + 3,4-dehydro-6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein + 3,4-dehydro-6-methoxymellein
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additional information
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S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein
S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein
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S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein
S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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biosynthetic pathway
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additional information
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no substrates: naringenin, apigenein
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additional information
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no substrates: umbelliferone, scopoletin
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additional information
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no substrates: umbelliferone, scopoletin
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additional information
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no substrates: 3,4-dehydro-6-methoxymellein, 6-methoxymellein, esculetin
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue
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S-adenosyl-L-methionine + 6-hydroxymellein
S-adenosyl-L-homocysteine + 6-methoxymellein
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biosynthetic pathway
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p-chloromercuribenzoate
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S-adenosyl-L-homocysteine
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2-Chloroethylphosphonic acid
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elicitor, the activity increases after a 2 day lag period and attains a maximum 3 days after the addition, then decreases gradually
Fusarium moniliforme endo-polygalacturonsae
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elicitor, the enzyme activity reaches a maximum 40 h after the addition, then decreased graduelly
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Phythophthora megasperma cell wall fraction
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elicitor, the enzyme activity reaches a maximum 50 h after the addition
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0.047
6-methoxymellein
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0.026
S-adenosyl-L-homocysteine
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additional information
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brenda
treated with 2-chloroethylphosphonic acid
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brenda
treated with fungal elicitors
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brenda
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brenda
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4°C, 7 d, complete loss of activity
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frozen overnight, complete loss of activity
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Kurosaki, F.; Nishi, A.
A methyltransferase forsynthesis of the phytoalexin 6-methoxymellein in carrot cells
FEBS Lett.
227
183-186
1988
Daucus carota
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brenda
Kurosaki, F.; Kizawa, Y.; Nishi, A.
Biosynthesis of dihydroisocoumarin by extracts of elicitor treated carrot root
Phytochemistry
28
1843-1845
1989
Daucus carota
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brenda
Kurosaki, F.
Partial purification and characterization of 6-hydroxymellein-O-methyltransferase from elicitor-treated carrot cells
Phytochemistry
41
1023-1027
1996
Daucus carota
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brenda
Marinelli, F.; Ronchi, V.N.; Salvadori, P.
Elicitor induction of enzyme activities and 6-methoxymellein production in carrot cell suspension culture
Phytochemistry
35
1457-1460
1994
Daucus carota
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brenda
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