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(R)-nicotine + acceptor + H2O
(R)-6-hydroxynicotine + reduced acceptor
-
-
-
?
(RS)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
(S)-nicotine + acceptor
(S)-nicotine delta1',5'-iminium ion + reduced acceptor
-
-
-
?
(S)-nicotine + acceptor + H2O
nicotine-N-oxide + reduced acceptor
-
-
-
?
(S)-nicotine + acceptor + H2O
nornicotine + reduced acceptor + ?
-
-
-
?
(S)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
(S)-nicotine + brilliant cresyl blue + H2O
(S)-6-hydroxynicotine + reduced brilliant cresyl blue
-
-
-
-
r
(S)-nicotine + menadione + H2O
(S)-6-hydroxynicotine + menadiol
-
-
-
-
r
(S)-nicotine + methylene blue + H2O
(S)-6-hydroxynicotine + reduced methylene blue
-
-
-
-
r
(S)-nicotine + pseudoazurin + H2O
(S)-6-hydroxynicotine + reduced pseudoazurin
(S)-nicotine + vitamin K5 + H2O
(S)-6-hydroxynicotine + reduced vitamin K5
-
-
-
-
r
anabasine + acceptor + H2O
6-hydroxyanabasine + reduced acceptor
-
-
-
?
D-nicotine + acceptor + H2O
(R)-6-hydroxynicotine + reduced acceptor
isozyme specific for the D-isomer
-
-
?
DL-nicotine + ?
6-hydroxynicotine + ?
-
first enzyme in nicotine pathway
-
?
L-nicotine + acceptor + H2O
(S)-6-hydroxynicotine + reduced acceptor
myosmine + acceptor + H2O
? + reduced acceptor
-
-
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
nicotine-N-oxide + acceptor + H2O
6-hydroxynicotine-N-oxide + reduced acceptor
-
-
-
?
nicotinic acid + acceptor + H2O
6-hydroxynicotinic acid + reduced acceptor
nornicotine + acceptor + H2O
6-hydroxynornicotine + reduced acceptor
additional information
?
-
(RS)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity
-
-
?
(RS)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity
-
-
?
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
-
r
(S)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity
-
-
?
(S)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity
-
-
?
(S)-nicotine + pseudoazurin + H2O
(S)-6-hydroxynicotine + reduced pseudoazurin
-
acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron
-
-
?
(S)-nicotine + pseudoazurin + H2O
(S)-6-hydroxynicotine + reduced pseudoazurin
-
acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron
-
-
?
L-nicotine + acceptor + H2O
(S)-6-hydroxynicotine + reduced acceptor
-
-
-
-
?
L-nicotine + acceptor + H2O
(S)-6-hydroxynicotine + reduced acceptor
-
-
-
-
?
L-nicotine + acceptor + H2O
(S)-6-hydroxynicotine + reduced acceptor
-
-
-
-
?
L-nicotine + acceptor + H2O
(S)-6-hydroxynicotine + reduced acceptor
isozyme strictly specific for the L-isomer
-
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
non-stereospecific enzyme if grown on L-nicotine, assayed with 2,6-dichlorophenolindophenol
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
brilliant cresyl blue, methylene blue, 5-hydroxy-1,4-naphthoquinone, 2,6-dichlorophenolindophenol, menadione and vitamin K5 used as electron acceptors
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
preferentially 2,6-dichlorophenolindophenol used as electron acceptor
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
molybdate is required
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
molybdate is required
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
first enzyme in the nicotine degradation pathway
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
first enzyme in the nicotine degradation pathway
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
-
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
-
-
?
nicotinic acid + acceptor + H2O
6-hydroxynicotinic acid + reduced acceptor
-
-
-
?
nicotinic acid + acceptor + H2O
6-hydroxynicotinic acid + reduced acceptor
-
cytochrome-linked oxidation, hydroxylation
-
?
nicotinic acid + acceptor + H2O
6-hydroxynicotinic acid + reduced acceptor
-
cytochrome-linked oxidation, hydroxylation
-
?
nicotinic acid + acceptor + H2O
6-hydroxynicotinic acid + reduced acceptor
-
2,6-dichlorophenolindophenol used as electron acceptor
-
?
nicotinic acid + acceptor + H2O
6-hydroxynicotinic acid + reduced acceptor
-
2,6-dichlorophenolindophenol used as electron acceptor
-
?
nornicotine + acceptor + H2O
6-hydroxynornicotine + reduced acceptor
-
-
-
?
nornicotine + acceptor + H2O
6-hydroxynornicotine + reduced acceptor
-
-
-
?
additional information
?
-
-
NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors
-
-
-
additional information
?
-
-
NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors
-
-
-
additional information
?
-
enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR
-
-
?
additional information
?
-
enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR
-
-
?
additional information
?
-
enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR
-
-
?
additional information
?
-
organism can grow on L-nicotine since it is converted to D-nicotine by a racemase
-
-
?
additional information
?
-
organism can grow on L-nicotine since it is converted to D-nicotine by a racemase
-
-
?
additional information
?
-
organism can grow on L-nicotine since it is converted to D-nicotine by a racemase
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-nicotine + acceptor + H2O
(R)-6-hydroxynicotine + reduced acceptor
-
-
-
?
(S)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
D-nicotine + acceptor + H2O
(R)-6-hydroxynicotine + reduced acceptor
isozyme specific for the D-isomer
-
-
?
DL-nicotine + ?
6-hydroxynicotine + ?
-
first enzyme in nicotine pathway
-
?
L-nicotine + acceptor + H2O
(S)-6-hydroxynicotine + reduced acceptor
isozyme strictly specific for the L-isomer
-
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
nornicotine + acceptor + H2O
6-hydroxynornicotine + reduced acceptor
-
-
-
?
additional information
?
-
(S)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity
-
-
?
(S)-nicotine + acceptor + H2O
pseudooxynicotine + reduced acceptor
via N-methylmyosmine, which then spontaneously hydrolyzes to pseudooxynicotine. The two enantiomers are degraded at approximately the same rate, indicating that NOX does not show chiral selectivity
-
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
first enzyme in the nicotine degradation pathway
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
first enzyme in the nicotine degradation pathway
-
?
nicotine + acceptor + H2O
6-hydroxynicotine + reduced acceptor
-
-
-
?
additional information
?
-
enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR
-
-
?
additional information
?
-
enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR
-
-
?
additional information
?
-
enzyme expression and activity is regulated by substrate and product level via transcription repressor HdnoR
-
-
?
additional information
?
-
organism can grow on L-nicotine since it is converted to D-nicotine by a racemase
-
-
?
additional information
?
-
organism can grow on L-nicotine since it is converted to D-nicotine by a racemase
-
-
?
additional information
?
-
organism can grow on L-nicotine since it is converted to D-nicotine by a racemase
-
-
?
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Nagel, M.; Koenig, K.; Andreesen, J.R.
Bactopterin as component of eubacterial dehydrogenases involved in hydroxylation reactions initiating the degradation of nicotine, nicotinate, and 2-furancarboxylate
FEMS Microbiol. Lett.
60
323-326
1989
Pseudarthrobacter oxydans
-
brenda
Schenk, S.; Hoelz, A.; Krauss, B.; Decker, K.
Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans
J. Mol. Biol.
284
1323-1339
1998
Paenarthrobacter nicotinovorans
brenda
Grether-Beck, S.; Igloi, G.L.; Pust, S.; Schilz, E.; Decker, K.; Brandsch, R.
Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans
Mol. Microbiol.
13
929-936
1994
Paenarthrobacter nicotinovorans (Q93NH4), Paenarthrobacter nicotinovorans
brenda
Decker, K.; Bleeg, H.
Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans
Biochim. Biophys. Acta
105
313-324
1965
Pseudarthrobacter oxydans
brenda
Freudenberg, W.; Koenig, K.; Andreesen, J.R.
Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase
FEMS Microbiol. Lett.
52
13-18
1988
Pseudarthrobacter oxydans
-
brenda
Hochstein, L.L.; Dalton, B.P.
The purification and properties of nicotine oxidase
Biochim. Biophys. Acta
139
56-68
1967
Pseudarthrobacter oxydans
brenda
Williams, D.E.; Ding, X.; Coon, M.J.
Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase
Biochem. Biophys. Res. Commun.
166
945-952
1990
Oryctolagus cuniculus
brenda
Nakayama, H.; Nakashima, T.; Kurogochi, Y.
Heterogeneity of hepatic nicotine oxidase
Biochim. Biophys. Acta
715
254-257
1982
Rattus norvegicus
brenda
Jones, M.V.
Cytochrome c linked nicotinic acid hydroxylase in Pseudomonas ovalis chester
FEBS Lett.
32
321-324
1973
Pseudomonas putida, Pseudomonas putida Chester
brenda
Behrman, E.J.; Stanier, R.Y.
The bacterial oxidation of nicotinic acid
J. Biol. Chem.
228
923-945
1957
Pseudomonas fluorescens, Pseudomonas fluorescens N-9
brenda
Igloi, G.L.; Brandsch, R.
Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system
J. Bacteriol.
185
1976-1986
2003
Paenarthrobacter nicotinovorans (Q59127), Paenarthrobacter nicotinovorans (Q59128), Paenarthrobacter nicotinovorans (Q59129)
brenda
Sandu, C.; Chiribau, C.B.; Brandsch, R.
Characterization of HdnoR, the transcriptional repressor of the 6-hydroxy-D-nicotine oxidase gene of Arthrobacter nicotinovorans pAO1, and its DNA-binding activity in response to L- and D-nicotine Derivatives
J. Biol. Chem.
278
51307-51315
2003
Paenarthrobacter nicotinovorans (Q59127), Paenarthrobacter nicotinovorans (Q59128), Paenarthrobacter nicotinovorans (Q59129)
brenda
Sachelaru, P.; Schiltz, E.; Brandsch, R.
A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans
Appl. Environ. Microbiol.
72
5126-5131
2006
Paenarthrobacter nicotinovorans
brenda
Ganas, P.; Sachelaru, P.; Mihasan, M.; Igloi, G.L.; Brandsch, R.
Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp. strain JS614
Arch. Microbiol.
189
511-517
2008
Paenarthrobacter nicotinovorans, Nocardioides sp.
brenda
Qiu, J.; Ma, Y.; Zhang, J.; Wen, Y.; Liu, W.
Cloning of a novel nicotine oxidase gene from Pseudomonas sp. strain HZN6 whose product nonenantioselectively degrades nicotine to pseudooxynicotine
Appl. Environ. Microbiol.
79
2164-2171
2013
no activity in Pseudomonas putida strain KT2440, no activity in Escherichia coli strain DH5alpha, Pseudomonas sp. (M4T5L3), Pseudomonas sp. HZN6 (M4T5L3)
brenda
Li, H.; Xie, K.; Yu, W.; Hu, L.; Huang, H.; Xie, H.; Wang, S.
Nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in Agrobacterium tumefaciens S33
Appl. Environ. Microbiol.
82
1745-1755
2016
Agrobacterium tumefaciens, Agrobacterium tumefaciens S33
brenda
Qiu, J.; Li, N.; Lu, Z.; Yang, Y.; Ma, Y.; Niu, L.; He, J.; Liu, W.
Conversion of nornicotine to 6-hydroxy-nornicotine and 6-hydroxy-myosmine by Shinella sp. strain HZN7
Appl. Microbiol. Biotechnol.
100
10019-10029
2016
Shinella sp. HZN7 (A0A0Y0MN26)
brenda
Yu, W.; Wang, R.; Huang, H.; Xie, H.; Wang, S.
Periplasmic nicotine dehydrogenase NdhAB utilizes pseudoazurin as its physiological electron acceptor in Agrobacterium tumefaciens S33
Appl. Environ. Microbiol.
83
e01050
2017
Agrobacterium tumefaciens, Agrobacterium tumefaciens S33
brenda