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IUBMB CommentsRequires Fe(II) and ascorbate. cf. EC 1.14.11.3, pyrimidine-deoxynucleoside 2'-dioxygenase.
The expected taxonomic range for this enzyme is: Rhodotorula glutinis
Synonyms
oxygenase, deoxyuridine-uridine 1'-di-,
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oxygenase, deoxyuridine-uridine 1'-di-
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2'-deoxyuridine + 2-oxoglutarate + O2 = uracil + 2-deoxyribonolactone + succinate + CO2
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oxidative decarboxylation
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2'-deoxyuridine,2-oxoglutarate:oxygen oxidoreductase (1'-hydroxylating)
Requires Fe(II) and ascorbate. cf. EC 1.14.11.3, pyrimidine-deoxynucleoside 2'-dioxygenase.
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2'-deoxy-2'-fluorouridine + 2-oxoglutarate + O2
uracil + 2-fluororibonolactone + succinate + CO2
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2'-deoxyuridine + 2-oxoglutarate + O2
uracil + deoxyribonolactone + succinate + CO2
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uridine + 2-oxoglutarate + O2
ribonolactone + uracil + succinate + CO2
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2'-deoxyuridine + 2-oxoglutarate + O2
uracil + deoxyribonolactone + succinate + CO2
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-20°C, stable for at least a month in 0.02 M sodium phosphate buffer, pH 7.5, with 0.1 mM EDTA
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Stubbe, J.
Identification of two alpha-ketoglutarate-dependent dioxygenases in extracts of Rhodotorula glutinis catalyzing deoxyuridine hydroxylation
J. Biol. Chem.
260
9972-9975
1985
Rhodotorula glutinis
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