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IUBMB Comments This membrane-bound, di-heme containing enzyme, identified in the bacterium Escherichia coli, is responsible for the detoxification of superoxide in the periplasm. In vivo the reaction proceeds in the opposite direction of that shown and produces oxygen. Superoxide production was only observed when the enzyme was incubated in vitro with an excess of ubiquinol.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
cytochrome b559, cyt b559, copper- and zinc-containing superoxide dismutase, superoxide oxidase,
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copper- and zinc-containing superoxide dismutase
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can also act as a superoxide reductase and a superoxide oxidase
O2- :Fe(III) oxidoreductase
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superoxide:ubiquinone oxidoreductase
cytochrome b561
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SOO
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superoxide:ubiquinone oxidoreductase
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superoxide:ubiquinone oxidoreductase
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2 O2 + ubiquinol = 2 superoxide + ubiquinone + 2 H+
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ubiquinol:oxygen oxidoreductase (superoxide-forming)
This membrane-bound, di-heme containing enzyme, identified in the bacterium Escherichia coli, is responsible for the detoxification of superoxide in the periplasm. In vivo the reaction proceeds in the opposite direction of that shown and produces oxygen. Superoxide production was only observed when the enzyme was incubated in vitro with an excess of ubiquinol.
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2 O2 + ubiquinol
2 superoxide + ubiquinone + 2 H+
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superoxide + Fe2+
O2 + Fe3+
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superoxide + ubiquinone Q2 + H+
O2 + ubiquinol Q2
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additional information
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additional information
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when the reconstituted cytochrome-containing vesicles were preloaded with ascorbic acid and cytochrome c is added to the external medium, the internal ascorbic acid is able to reduce the external cytochrome c. This reduction of cytochrome c is dependent on the presence of the enzyme in the membrane
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additional information
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the enzyme does not bind CO
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no activities with ferrocyanide or ferricyanide as the redox partners
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2 O2 + ubiquinol
2 superoxide + ubiquinone + 2 H+
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superoxide + Fe2+
O2 + Fe3+
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heme
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diheme cytochrome b
heme
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the diheme cytochrome contains 91.2 nmol of heme per mg of protein
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Amyotrophic Lateral Sclerosis
Copper- and zinc-containing superoxide dismutase can act as a superoxide reductase and a superoxide oxidase.
Cataract
Prevalence of cataract in adult Down's syndrome patients aged 28 to 83 years.
Cholesteatoma
Cathepsin L activity and its inhibitor in human otitis media.
Dehydration
Effect of dehydration on light-induced reactions in photosystem II: photoreactions of cytochrome b559.
Granulomatous Disease, Chronic
Deficient flavoprotein component of the NADPH-dependent O2-.-generating oxidase in the neutrophils from three male patients with chronic granulomatous disease.
Granulomatous Disease, Chronic
Recombinant interferon gamma augments phagocyte superoxide production and X-chronic granulomatous disease gene expression in X-linked variant chronic granulomatous disease.
Infections
Active copper- and zinc-containing superoxide dismutase in the cryptic genospecies of Haemophilus causing urogenital and neonatal infections discriminates them from Haemophilus influenzae sensu stricto.
Neoplasms
Anticancer therapy by overexpression of superoxide dismutase.
Neoplasms
Copper- and zinc-containing superoxide dismutase and manganese-containing superoxide dismutase in human tissues and human malignant tumors.
Neoplasms
Pathological Study of Acute Pulmonary Toxicity Induced by Intratracheally Instilled Asian Sand Dust (Kosa).
Neoplasms
Production of large amounts of hydrogen peroxide by human tumor cells.
Neoplasms
v-Ha-ras mitogenic signaling through superoxide and derived reactive oxygen species.
Otitis Media
Cathepsin L activity and its inhibitor in human otitis media.
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9.6
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isoelectric focusing
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integral membrane protein
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metabolism
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the enzyme catalyzes a transmembrane electron transfer
metabolism
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the enzyme functions as an intermediary electron-transferring component before ubiquinone in the respiratory chain of Escherichia coli K-12
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His-selenomethionine-labelled enzyme, sitting drop vapor diffusion method, using 200 mM HEPES pH 7.5, 5% (v/v) glycerol, 25% (w/v) PEG 2000 MME, 50 mM MgCl2
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DEAE-Sephacel column chromatography, CM-Sepharose CL-GB column chromatography, and Sephacryl S-200 gel filtration
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Ni2+-charged Profinity IMAC resin column chromatography and Biosep-Sec-S3000 gel filtration
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expressed in Escherichia coli BL21/pLysS cells
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Tiwari, A.; Pospisil, P.
Superoxide oxidase and reductase activity of cytochrome b559 in photosystem II
Biochim. Biophys. Acta
1787
985-994
2009
Spinacia oleracea
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Srivastava, M.; Duong, L.T.; Fleming, P.J.
Cytochrome b561 catalyzes transmembrane electron transfer
J. Biol. Chem.
259
8072-8075
1984
Bos taurus
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Murakami, H.; Kita, K.; Anraku, Y.
Purification and properties of a diheme cytochrome b561 of the Escherichia coli respiratory chain
J. Biol. Chem.
261
548-551
1986
Escherichia coli
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Liochev, S.I.; Fridovich, I.
Copper- and zinc-containing superoxide dismutase can act as a superoxide reductase and a superoxide oxidase
J. Biol. Chem.
275
38482-38485
2000
Homo sapiens
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Murakami, H.; Kita, K.; Anraku, Y.
Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12
Mol. Gen. Genet.
198
1-6
1984
Escherichia coli
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Lundgren, C.A.K.; Sjoestrand, D.; Biner, O.; Bennett, M.; Rudling, A.; Johansson, A.L.; Brzezinski, P.; Carlsson, J.; von Ballmoos, C.; Hoegbom, M.
Scavenging of superoxide by a membrane-bound superoxide oxidase
Nat. Chem. Biol.
14
788-793
2018
Escherichia coli
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