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D-(-)fructose + 2,6-dichloroindophenol
5-dehydro-D-(-)-fructose + reduced 2,6-dichloroindophenol
D-(-)fructose + cytochrome c
5-dehydro-D-(-)-fructose + reduced cytochrome c
D-(-)fructose + potassium ferricyanide
5-dehydro-D-(-)-fructose + potassium ferrocyanide
D-fructose + 1,1'-dimethylferricenium ion
5-dehydro-D-fructose + 1,1'-dimethylferrocenium ion
-
-
-
-
?
D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone
5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol
D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
D-fructose + 2,5-bis[methyl(phenyl)amino]cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2,5-bis[methyl(phenyl)amino]benzene-1,4-diol
-
-
-
-
?
D-fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
D-fructose + 2-(2-fluoroanilino)cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2-(2-fluoroanilino)benzene-1,4-diol
-
-
-
-
?
D-fructose + 2-(2-methoxyanilino)cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2-(2-methoxyanilino)benzene-1,4-diol
-
-
-
-
?
D-fructose + 2-(3-nitroanilino)cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2-(3-nitroanilino)benzene-1,4-diol
-
-
-
-
?
D-fructose + 2-(4-fluoroanilino)cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2-(4-fluoroanilino)benzene-1,4-diol
-
-
-
-
?
D-fructose + 2-(4-methoxyanilino)cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2-(4-methoxyanilino)benzene-1,4-diol
-
-
-
-
?
D-fructose + 2-[methyl(phenyl)amino]cyclohexa-2,5-diene-1,4-dione
5-dehydro-D-fructose + 2-[methyl(phenyl)amino]benzene-1,4-diol
-
-
-
-
?
D-fructose + 7,7,8,8-tetracyanoquinodimethane
5-dehydro-D-fructose + reduced 7,7,8,8-tetracyanoquinodimethane
-
-
-
-
?
D-fructose + acceptor
2-dehydro-D-fructose + reduced acceptor
-
direct electron-transfer bioelectrocatalytic oxidation, DET-type
-
-
?
D-fructose + acceptor
5-dehydro-D-fructose + reduced acceptor
D-fructose + an ubiquinone
5-dehydro-D-fructose + an ubiquinol
D-fructose + electron acceptor
5-dehydro-D-fructose + reduced electron acceptor
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
D-fructose + nitro blue tetrazolium
5-dehydro-D-fructose + ?
D-fructose + oxidized 1-methoxy-5-methylphenazinium methylsulfate
5-dehydro-D-fructose + reduced 1-methoxy-5-methylphenazinium methylsulfate
-
-
-
-
?
D-fructose + oxidized electron acceptor
5-dehydro-D-fructose + reduced electron acceptor
D-fructose + phenazine methosulfate
5-dehydro-D-fructose + ?
D-fructose + potassium ferricyanide
5-dehydro-D-fructose + potassium ferrocyanide
D-fructose + ubiquinone
5-dehydro-D-fructose + ubiquinol
D-fructose + [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]
5-dehydro-D-fructose + [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] formazan
-
in the presence of phenazine methosulfate
-
-
?
D-fructose + [Fe(CN)6]3-
5-dehydro-D-fructose + [Fe(CN)6]4-
-
-
-
-
?
fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
fructose + an ubiquinone
5-dehydro-D-fructose + an ubiquinol
fructose + phenazine methosulfate
5-dehydro-D-fructose + reduced phenazine methosulfate
additional information
?
-
D-(-)fructose + 2,6-dichloroindophenol
5-dehydro-D-(-)-fructose + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-(-)fructose + 2,6-dichloroindophenol
5-dehydro-D-(-)-fructose + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-(-)fructose + cytochrome c
5-dehydro-D-(-)-fructose + reduced cytochrome c
-
-
-
-
?
D-(-)fructose + cytochrome c
5-dehydro-D-(-)-fructose + reduced cytochrome c
-
-
-
-
?
D-(-)fructose + potassium ferricyanide
5-dehydro-D-(-)-fructose + potassium ferrocyanide
-
-
-
-
?
D-(-)fructose + potassium ferricyanide
5-dehydro-D-(-)-fructose + potassium ferrocyanide
-
-
-
-
?
D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone
5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol
-
-
-
-
?
D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinone
5-dehydro-D-fructose + 2,3-dimethoxy-5-farnesyl-1,4-benzoquinol
-
-
-
-
?
D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
-
-
-
?
D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
5-dehydro-D-fructose + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
-
-
-
?
D-fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
most effective electron acceptor
-
-
?
D-fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-fructose + acceptor
5-dehydro-D-fructose + reduced acceptor
-
-
-
-
?
D-fructose + acceptor
5-dehydro-D-fructose + reduced acceptor
-
-
-
-
?
D-fructose + acceptor
5-dehydro-D-fructose + reduced acceptor
-
involved in utilization of D-fructose by acetic acid bacteria
-
-
?
D-fructose + acceptor
5-dehydro-D-fructose + reduced acceptor
-
-
-
-
?
D-fructose + an ubiquinone
5-dehydro-D-fructose + an ubiquinol
-
-
-
-
?
D-fructose + an ubiquinone
5-dehydro-D-fructose + an ubiquinol
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
-
?
D-fructose + ferricyanide
5-dehydro-D-fructose + ferrocyanide
-
-
-
-
?
D-fructose + nitro blue tetrazolium
5-dehydro-D-fructose + ?
-
-
-
?
D-fructose + nitro blue tetrazolium
5-dehydro-D-fructose + ?
-
-
-
?
D-fructose + oxidized electron acceptor
5-dehydro-D-fructose + reduced electron acceptor
-
-
-
-
?
D-fructose + oxidized electron acceptor
5-dehydro-D-fructose + reduced electron acceptor
-
-
-
-
?
D-fructose + phenazine methosulfate
5-dehydro-D-fructose + ?
-
-
-
?
D-fructose + phenazine methosulfate
5-dehydro-D-fructose + ?
-
-
-
?
D-fructose + potassium ferricyanide
5-dehydro-D-fructose + potassium ferrocyanide
-
-
-
-
?
D-fructose + potassium ferricyanide
5-dehydro-D-fructose + potassium ferrocyanide
-
-
-
-
?
D-fructose + potassium ferricyanide
5-dehydro-D-fructose + potassium ferrocyanide
-
-
-
-
?
D-fructose + potassium ferricyanide
5-dehydro-D-fructose + potassium ferrocyanide
-
-
-
-
?
D-fructose + ubiquinone
5-dehydro-D-fructose + ubiquinol
-
-
-
-
?
D-fructose + ubiquinone
5-dehydro-D-fructose + ubiquinol
-
-
-
-
?
fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
fructose + 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
fructose + an ubiquinone
5-dehydro-D-fructose + an ubiquinol
-
-
-
-
?
fructose + an ubiquinone
5-dehydro-D-fructose + an ubiquinol
-
-
-
-
?
fructose + phenazine methosulfate
5-dehydro-D-fructose + reduced phenazine methosulfate
-
-
-
-
?
fructose + phenazine methosulfate
5-dehydro-D-fructose + reduced phenazine methosulfate
-
-
-
-
?
additional information
?
-
-
no activity with NADP+
-
-
?
additional information
?
-
-
no activity with NAD+
-
-
?
additional information
?
-
-
oxygen is completely inactive as an electron acceptor
-
-
?
additional information
?
-
-
when FDH is adsorbed onto a porous carbon electrode surface, it can transfer electrons from D-fructose to the electrode directly without a mediator
-
-
?
additional information
?
-
-
cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction
-
-
?
additional information
?
-
-
subunit II is equivalent to the cytochrome domain and acts as electron acceptor to the catalytic subunit I of the heterotrimeric enzyme
-
-
-
additional information
?
-
-
cells producing only subunits FdhS and FdhL have no fructose-oxidizing activity, but show significantly high D-fructose:ferricyanide oxidoreductase activity in the soluble fraction of cell extracts, whereas the cells producing the FDH complex show activity in the membrane fraction
-
-
?
additional information
?
-
-
subunit II is equivalent to the cytochrome domain and acts as electron acceptor to the catalytic subunit I of the heterotrimeric enzyme
-
-
-
additional information
?
-
-
no activity with NADP+
-
-
?
additional information
?
-
-
no activity with NADP+
-
-
?
additional information
?
-
-
no activity with O2
-
-
?
additional information
?
-
-
no activity with O2
-
-
?
additional information
?
-
-
no activity with NAD+
-
-
?
additional information
?
-
-
no activity with NAD+
-
-
?
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Ameyama, M.; Adachi, O.
D-Fructose dehydrogenase from Gluconobacter industrius, membrane-bound
Methods Enzymol.
89
154-159
1982
Gluconobacter oxydans
-
brenda
Yamada, Y.; Aida, K.; Uemura, T.
Enzymatic studies on the oxidation of sugar and sugar alcohol. I. Purification and properties of particle-bound fructose dehydrogenase
J. Biochem.
61
636-646
1967
Gluconobacter cerinus
brenda
Ameyama, M.; Shinagawa, E.; Matsushita, K.; Adachi, O.
D-Fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose
J. Bacteriol.
145
814-823
1981
Gluconobacter oxydans
brenda
Marcinkeviciene, J.; Johansson, G.
Kinetic studies of the active sites functioning in the quinohemoprotein fructose dehydrogenase
FEBS Lett.
318
23-26
1993
Gluconobacter sp.
brenda
Kato, K.; Walde, P.; Mitsui, H.; Higashi, N.
Enzymatic activity and stability of D-fructose dehydrogenase and sarcosine dehydrogenase immobilized onto giant vesicles
Biotechnol. Bioeng.
84
415-423
2003
Gluconobacter sp.
brenda
Kamitaka, Y.; Tsujimura, S.; Kano, K.
High current density bioelectrolysis of D-fructose at fructose dehydrogenase-adsorbed and Ketjen black-modified electrodes without a mediator
Chem. Lett.
36
218-219
2007
Gluconobacter sp.
-
brenda
Tominaga, M.; Nomura, S.; Taniguchi, I.
D-fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode
Biosens. Bioelectron.
24
1184-1188
2009
Gluconobacter sp.
brenda
Kimata, S.; Mizuguchi, K.; Hattori, S.; Teshima, S.; Orita, Y.
Evaluation of a new automated, enzymatic inulin assay using D-fructose dehydrogenase
Clin. Exp. Nephrol.
13
341-349
2009
Gluconobacter oxydans
brenda
Hui, H.; Huang, D.; McArthur, D.; Nissen, N.; Boros, L.G.; Heaney, A.P.
Direct spectrophotometric determination of serum fructose in pancreatic cancer patients
Pancreas
38
706-712
2009
Gluconobacter sp.
brenda
Tsujimura, S.; Nishina, A.; Kamitaka, Y.; Kano, K.
Coulometric D-fructose biosensor based on direct electron transfer using D-fructose dehydrogenase
Anal. Chem.
81
9383-9387
2009
Gluconobacter frateurii
brenda
Sasaki, Y.; Sugihara, T.; Osakai, T.
Electron transfer mediated by membrane-bound D-fructose dehydrogenase adsorbed at an oil/water interface
Anal. Biochem.
417
129-135
2011
Gluconobacter sp.
brenda
Kawai, S.; Goda-Tsutsumi, M.; Yakushi, T.; Kano, K.; Matsushita, K.
Heterologous overexpression and characterization of a flavoprotein-cytochrome c complex fructose dehydrogenase of Gluconobacter japonicus NBRC3260
Appl. Environ. Microbiol.
79
1654-1660
2013
Gluconobacter japonicus, Gluconobacter japonicus NBRC3260
brenda
Sasakura, K.; Shirai, O.; Hichiri, K.; Goda-Tsutsumi, M.; Tsujimura, S.; Kano, K.
Ion transport across planar bilayer lipid membrane driven by D-fructose dehydrogenase-catalyzed electron transport
Chem. Lett.
40
486-488
2011
Gluconobacter sp.
-
brenda
Hickey, D.; Giroud, F.; Schmidtke, D.; Glatzhofer, D.; Minteer, S.
Enzyme cascade for catalyzing sucrose oxidation in a biofuel cell
ACS Catal.
3
2729-2737
2013
Gluconobacter sp.
-
brenda
Sarauli, D.; Wettstein, C.; Peters, K.; Schulz, B.; Fattakhova-Rohlfing, D.; Lisdat, F.
Interaction of fructose dehydrogenase with a sulfonated polyaniline: application for enhanced bioelectrocatalysis
ACS Catal.
5
2081-2087
2015
Gluconobacter japonicus
-
brenda
Wettstein, C.; Kano, K.; Schfer, D.; Wollenberger, U.; Lisdat, F.
Interaction of flavin-dependent fructose dehydrogenase with cytochrome c as basis for the construction of biomacromolecular architectures on electrodes
Anal. Chem.
88
6382-6389
2016
Gluconobacter japonicus, Gluconobacter japonicus NBRCA3260
brenda
Kawai, S.; Yakushi, T.; Matsushita, K.; Kitazumi, Y.; Shirai, O.; Kano, K.
The electron transfer pathway in direct electrochemical communication of fructose dehydrogenase with electrodes
Electrochem. Commun.
38
28-31
2014
Gluconobacter japonicus, Gluconobacter japonicus NBRC3260
-
brenda
Funabashi, H.; Murata, K.; Tsujimura, S.
Effect of pore size of MgO-templated carbon on the direct electrochemistry of D-fructose dehydrogenase
Electrochemistry
83
372-375
2015
Gluconobacter japonicus
-
brenda
Hichiri, K.; Shirai, O.; Kitazumi, Y.; Kano, K.
Coupling of proton transport across planar lipid bilayer and electron transport catalyzed by membrane-bound enzyme D-fructose dehydrogenase
Electrochemistry
84
328-333
2016
Gluconobacter japonicus
-
brenda
Kawai, S.; Yakushi, T.; Matsushita, K.; Kitazumi, Y.; Shirai, O.; Kano, K.
Role of a non-ionic surfactant in direct electron transfer-type bioelectrocatalysis by fructose dehydrogenase
Electrochim. Acta
152
19-24
2015
Gluconobacter japonicus
-
brenda
Sugimoto, Y.; Kitazumi, Y.; Shirai, O.; Yamamoto, M.; Kano, K.
Role of 2-mercaptoethanol in direct electron transfer-type bioelectrocatalysis of fructose dehydrogenase at Au electrodes
Electrochim. Acta
170
242-247
2015
Gluconobacter sp.
-
brenda
Sugimoto, Y.; Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.
Function of C-terminal hydrophobic region in fructose dehydrogenase
Electrochim. Acta
176
976-981
2015
Gluconobacter oxydans, Gluconobacter oxydans 621H
-
brenda
Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.
Bioelectrochemical characterization of the reconstruction of heterotrimeric fructose dehydrogenase from its subunits
Electrochim. Acta
210
689-694
2016
Gluconobacter japonicus, Gluconobacter japonicus NBRC3260
-
brenda
Xia, H.; Hibino, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.
Interaction between D-fructose dehydrogenase and methoxy-substituent-functionalized carbon surface to increase productive orientations
Electrochim. Acta
218
41-46
2016
Gluconobacter japonicus, Gluconobacter japonicus NBRC3260
-
brenda
Bollella, P.; Hibino, Y.; Kano, K.; Gorton, L.; Antiochia, R.
The influence of pH and divalent/monovalent cations on the internal electron transfer (IET), enzymatic activity, and structure of fructose dehydrogenase
Anal. Bioanal. Chem.
410
3253-3264
2018
Gluconobacter japonicus, Gluconobacter japonicus NCBR 3260
brenda
Bollella, P.; Hibino, Y.; Kano, K.; Gorton, L.; Antiochia, R.
Highly sensitive membraneless fructose biosensor based on fructose dehydrogenase immobilized onto aryl thiol modified highly porous gold electrode characterization and application in food samples
Anal. Chem.
90
12131-12136
2018
Gluconobacter japonicus, Gluconobacter japonicus NCBR 3260
brenda
Adachi, T.; Kaida, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.
Bioelectrocatalytic performance of D-fructose dehydrogenase
Bioelectrochemistry
129
1-9
2019
Gluconobacter japonicus
brenda
Siepenkoetter, T.; Salaj-Kosla, U.; Magner, E.
The immobilization of fructose dehydrogenase on nanoporous gold electrodes for the detection of fructose
ChemElectroChem
4
905-912
2017
Gluconobacter sp.
-
brenda
Voitechovic, E.; Vektariene, A.; Vektaris, G.; Janciene, R.; Razumiene, J.; Gureviciene, V.
1,4-Benzoquinone derivatives for enhanced bioelectrocatalysis by fructose dehydrogenase from Gluconobacter japonicus Towards promising D-fructose biosensor development
Electroanalysis
32
1005-1016
2020
Gluconobacter japonicus
-
brenda
Hibino, Y.; Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.
Construction of a protein-engineered variant of D-fructose dehydrogenase for direct electron transfer-type bioelectrocatalysis
Electrochem. Commun.
77
112-115
2017
Gluconobacter japonicus, Gluconobacter japonicus NCBR 3260
-
brenda
Kaida, Y.; Hibino, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.
Ultimate downsizing of D-fructose dehydrogenase for improving the performance of direct electron transfer-type bioelectrocatalysis
Electrochem. Commun.
98
101-105
2019
Gluconobacter japonicus, Gluconobacter japonicus NBRC3260
-
brenda
Hibino, Y.; Kawai, S.; Kitazumi, Y.; Shirai, O.; Kano, K.
Protein-engineering improvement of direct electron transfer-type bioelectrocatalytic properties of D-fructose dehydrogenase
Electrochemistry
87
47-51
2019
Gluconobacter japonicus, Gluconobacter japonicus NBRC 3260
-
brenda
Kaida, Y.; Hibino, Y.; Kitazumi, Y.; Shirai, O.; Kano, K.
Discussion on direct electron transfer-type bioelectrocatalysis of downsized and axial-ligand exchanged variants of D-fructose dehydrogenase
Electrochemistry
88
195-199
2020
Gluconobacter japonicus, Gluconobacter japonicus NBRC3260
-
brenda
Hoffmann, J.; Hoevels, M.; Kosciow, K.; Deppenmeier, U.
Synthesis of the alternative sweetener 5-ketofructose from sucrose by fructose dehydrogenase and invertase producing Gluconobacter strains
J. Biotechnol.
307
164-174
2020
Gluconobacter japonicus, Gluconobacter japonicus LMG 1281
brenda
Suzuki, Y.; Kano, K.; Shirai, O.; Kitazumi, Y.
Diffusion-limited electrochemical D-fructose sensor based on direct electron transfer-type bioelectrocatalysis by a variant of D-fructose dehydrogenase at a porous gold microelectrode
J. Electroanal. Chem.
877
114651
2020
Gluconobacter japonicus, Gluconobacter japonicus NBRC 3260
-
brenda
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