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D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
-
-
-
-
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
B-type enzyme with regard to stereochemistry, transfers hydrogen from carbon-1 of D-galactose to si face of NAD, in the reverse reaction the pro-S hydrogen atom from carbon-4 of NAD(P)H is transferred to the re face of carbon-1 of D-galactono-1,5-lactone
-
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
B-type enzyme with regard to stereochemistry, transfers hydrogen from carbon-1 of D-galactose to si face of NAD, in the reverse reaction the pro-S hydrogen atom from carbon-4 of NAD(P)H is transferred to the re face of carbon-1 of D-galactono-1,5-lactone
-
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
depending on the pH, the immediately formed D-galactono-1,5-lactone rearranges intramolecularly to the corresponding 1,4-lactone or hydrolyzes to D-galctonate or both
-
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
depending on the pH, the immediately formed D-galactono-1,5-lactone rearranges intramolecularly to the corresponding 1,4-lactone or hydrolyzes to D-galctonate or both
-
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2-deoxy-D-galactose + NAD+
2-deoxy-galactono-1,5-lactone + NADH
2-deoxy-D-galactose + NADP+
2-deoxy-galactono-1,5-lactone + NADPH
-
-
-
?
3-deoxy-D-galactose + NAD+
3-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
4-deoxy-D-galactose + NAD+
4-deoxy-galactono-1,5-lactone + NAD(P)H
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
D-galactopyranose + NAD(P)+
D-galactono-1,5-lactone + NAD(P)H
-
-
-
-
?
D-galactose + NAD+
D-galactono-1,4-lactone + NADH + H+
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
succinate + NAD(P)+
succinic acid anhydride + NAD(P)H
-
-
-
?
2-deoxy-D-galactose + NAD+
2-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
2-deoxy-D-galactose + NAD+
2-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
2-deoxy-D-galactose + NAD+
2-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
6-deoxy-alpha-D-galactose + NAD+
6-deoxy-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,4-lactone + NADH + H+
-
-
-
-
?
D-galactose + NAD+
D-galactono-1,4-lactone + NADH + H+
-
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
-
-
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
-
-
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
1 mM, better substrate than D-galactose
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
-
-
-
?
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D-galactose + NAD+
D-galactono-1,4-lactone + NADH + H+
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
D-galactose + NAD+
D-galactono-1,4-lactone + NADH + H+
-
-
-
-
?
D-galactose + NAD+
D-galactono-1,4-lactone + NADH + H+
-
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NAD+
D-galactono-1,5-lactone + NADH
-
-
-
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
-
-
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
-
-
?
D-galactose + NADP+
D-galactono-1,5-lactone + NADPH
-
-
-
?
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0.0074
-
substrate combination L-arabinose and D-galactose, coenzyme NADP+, cell free extracts
0.008
-
substrate succinate, coenzyme NADP+, cell free extracts
0.009
-
crude extracts, cells grown on D-galactose, inducible enzyme
0.012
-
substrate succinate, coenzyme NAD+, cell free extracts
0.023
-
crude extracts, cells grown on D-galactose, inducible enzyme
0.026
-
crude extracts, cells grown on D-galactose, inducible enzyme
0.031
-
coenzyme NADP+, cell free extracts
0.036
-
coenzyme NAD+, cell free extracts
0.06
-
crude extracts, cells grown on sucrose
0.071
-
substrate combination L-arabinose and galactose, coenzyme NAD, cell free extracts
0.078
-
substrate L-arabinose, coenzyme NADP+, cell free extracts
0.079
-
substrate L-arabinose, coenzyme NAD+, cell free extracts
17
-
beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker
27
-
beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker
29
-
beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker
390
-
substrate L-arabinose
76
-
beta-galactosidase-galactose dehydrogenase fusion enzyme
76
-
substrate L-arabinose
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Kurn, N.; Contreras, I.; Shapiro, L.
Galactose catabolism in Caulobacter crescentus
J. Bacteriol.
135
517-520
1978
Caulobacter crescentus AE22, Caulobacter crescentus AE23, Caulobacter crescentus CB13
brenda
Arias, A.; Cervenansky, C.
Galactose metabolism in Rhizobium meliloti L5-30
J. Bacteriol.
167
1092-1094
1986
Sinorhizobium meliloti, Rhizobium meliloti L5-30, Sinorhizobium meliloti UR23
brenda
Hu, A.S.L.; Cline, A.L.
The regulation of some sugar dehydrogenases in a Pseudomonad
Biochim. Biophys. Acta
93
237-245
1964
Pseudomonas sp., Pseudomonas sp. G6
brenda
Carlsson, H.; Ljung, S.; Bulow, L.
Physical and kinetic effects on introduction of various linker regions in beta-galactosidase/galactose dehydrogenase fusion enzymes
Biochim. Biophys. Acta
1293
154-160
1996
Pseudomonas fluorescens
brenda
Carlsson, H.; Ljungcrantz, P.; Lindbladh, C.; Persson, M.; Bulow, L.
Use of geneticallly prepared enzyme conjugates in lactose and galactose analyses
Anal. Biochem.
218
278-283
1994
Pseudomonas fluorescens
brenda
Wong, T.Y.; Yao, X.T.
The deLey-Doudoroff pathway of galactose metabolism in Azotobacter vinelandii
Appl. Environ. Microbiol.
60
2065-2068
1994
Azotobacter vinelandii, Pseudomonas fluorescens, Pelomonas saccharophila, Rhizobium meliloti L5-30
brenda
Lindbladh, C.; Persson, M.; Bulow, L.; Mosbach, K.
Characterization of a recombinant bifunctional enzyme, galactose dehydrogenase/bacterial luciferase, displaying an improved bioluminescence in a three-enzyme system
Eur. J. Biochem.
204
241-247
1992
Pseudomonas fluorescens
brenda
Ljungcrantz, P.; Bulow, L.; Mosbach, K.
Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/beta-galactosidase/galactokinase
FEBS Lett.
275
91-94
1990
Pseudomonas fluorescens
brenda
Fiedler, S.; Buckel, P.
The D-galactose dehydrogenase gene from Pseudomonas fluorescens: characterization of mutations leading to increased expression in Escherichia coli
Appl. Microbiol. Biotechnol.
33
418-423
1990
Pseudomonas fluorescens
brenda
Fjimura, Y.
Fluorometric method with galactose dehydrogenase
Methods Enzymol.
6
288-296
1984
Pseudomonas fluorescens
-
brenda
Maier, E.; Kurz, G.
D-Galactose dehydrogenase from Pseudomonas fluorescens
Methods Enzymol.
89
176-181
1982
Pseudomonas fluorescens
brenda
Buckel, P.; Zehelein, E.
Expression of Pseudomonas fluorescens D-galactose dehydrogenase in E. coli
Gene
16
149-159
1981
Pseudomonas fluorescens
brenda
Brendel, K.; Bressler, R.; Alizade, M.A.
Stereochemistry of the hydrogen transfer to NAD catalyzed by D-galactose dehydrogenase from Pseudomonas fluorescens
Biochim. Biophys. Acta
397
1-4
1975
Pseudomonas fluorescens
brenda
Ueberschär, K.H.; Blachnitzky, E.O.; Lehman, J.; Kurz, G.
Stereospecificity of hydrogen transfer catalyzed by D-galactose dehydrogenase from Pseudomonas saccharophila and Pseudomonas fluorescens
Biochim. Biophys. Acta
391
15-18
1975
Pseudomonas fluorescens, Pelomonas saccharophila
brenda
Wengenmayer, F.; Kurz, G.
Subunit structure of D-galactose dehydrogenase from Pseudomonas Saccharophila
Biochim. Biophys. Acta
386
590-602
1975
Pelomonas saccharophila
brenda
Kurz, G.; Wallenfels, K.
UV-Test mit Galactose Dehydrogenase
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
1324-1327
1974
Pseudomonas fluorescens, Pelomonas saccharophila
-
brenda
Ueberschär, K.H.; Blachnitzky, E.O.; Kurz, G.
Reaction mechanism of D-galactose dehydrogenases from Pseudomonas saccharophila and Pseudomonas fluorescens
Eur. J. Biochem.
48
389-405
1974
Pseudomonas fluorescens, Pelomonas saccharophila
brenda
Blachnitzky, E.O.; Wengenmayer, F.; Kurz, G.
D-galactose dehydrogenase from Pseudomonas fluorescens. Purification, properties and structure
Eur. J. Biochem.
47
235-250
1974
Pseudomonas fluorescens, Pelomonas saccharophila
brenda
Wengenmayer, F.; Ueberschär, K.H.; Kurz, G.
Sulfhydryl group reactivity of D-galactose dehydrogenase from Pseudomonas saccharophila
Eur. J. Biochem.
43
49-58
1974
Pelomonas saccharophila
brenda
Wengenmayer, f.; Ueberschär, K.H.; Kurz, G.
D-Galactose dehydrogenase from Pseudomonas saccharophila. Purification, properties and structure
Eur. J. Biochem.
40
49-61
1973
Pelomonas saccharophila
brenda
Wengenmayer, F.; Blachnitzky, E.O.; Kurz, G.
Artefakt-Formen der D-Galaktose-Dehydrogenase
Hoppe-Seyler's Z. Physiol. Chem.
354
131-135
1973
Pelomonas saccharophila
brenda
Mazitsos, C.F.; Rigden, D.J.; Tsoungas, P.G.; Clonis, Y.D.
Galactosyl-mimodye ligands for Pseudomonas fluorescens beta-galactose dehydrogenase
Eur. J. Biochem.
269
5391-5405
2002
Pseudomonas fluorescens
brenda
Azar, S.R.; Naiebi, R.; Homami, A.; Akbari, Z.; Kianmehr, A.; Mahdizadehdehosta, R.; Najafzadeh, F.
Expression and response surface optimization of the recovery and purification of recombinant D-galactose dehydrogenase from Pseudomonas fluorescens
Indian J. Biochem. Biophys.
52
68-74
2015
Pseudomonas fluorescens
brenda
Shahbaz Mohammadi, H.; Mostafavi, S.S.; Soleimani, S.; Bozorgian, S.; Pooraskari, M.; Kianmehr, A.
Response surface methodology to optimize partition and purification of two recombinant oxidoreductase enzymes, glucose dehydrogenase and d-galactose dehydrogenase in aqueous two-phase systems
Protein Expr. Purif.
108
41-47
2015
Pseudomonas fluorescens, Pseudomonas fluorescens AK92
brenda