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EC Tree
IUBMB Comments The enzyme is involved in pheromone production by the pine engraver beetle, Ips pini.
The expected taxonomic range for this enzyme is: Ips pini
Synonyms
idoldh, ipsdienol dehydrogenase,
more
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(R)-ipsdienol + NAD(P)+ = ipsdienone + NAD(P)H + H+
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(R)-ipsdienol:NAD(P)+ oxidoreductase
The enzyme is involved in pheromone production by the pine engraver beetle, Ips pini.
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(4R)-ipsdienol + NAD(P)+
ipsdienone + NAD(P)H + H+
the enzyme is involved in pheromon production
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(4R)-ipsdienol + NAD+
ipsdienone + NADH + H+
no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+
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(4R)-ipsdienol + NADP+
ipsdienone + NADPH + H+
no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+
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(R)-ipsdienol + NADP+
ipsdienone + NADPH + H+
ipsdienone + NADPH + H+
(R)-ipsdienol + NADP+
stereospecific reaction
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r
ipsenone + NADPH + H+
racemic ipsenol + NADP+
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r
racemic ipsenol + NADP+
ipsenone + NADPH + H+
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r
additional information
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(R)-ipsdienol + NADP+
ipsdienone + NADPH + H+
stereospecific reaction
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r
(R)-ipsdienol + NADP+
ipsdienone + NADPH + H+
stereospecific reaction, no activity with (S)-ipsdienol. The oxidation of (R)-ipsdienol is 6.3times higher than that for the reduction of ipsdienone
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r
additional information
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the enzyme stereospecifically reduces both ketones to their corresponding (R)-alcohols. Low activity with (R)-menthone, 3-penten-2-ol, 17beta-estradiol, poor or no activity with (S)-ipsdienol, nerol, geraniol, myrcene, citral, 4-methyl-3-penten-2-one, 4-methyl-2-pentanone, 4-methyl-2-pentanol, acetone, 1,3-butendiol, etanol, 2-propanol, 3-hydroxybutyrate, and 2-oxobutyrate, substrate specificity, overview. Product identification by GC-MS analysis
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additional information
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the enzyme stereospecifically reduces both ketones to their corresponding (R)-alcohols. Low activity with (R)-menthone, 3-penten-2-ol, 17beta-estradiol, poor or no activity with (S)-ipsdienol, nerol, geraniol, myrcene, citral, 4-methyl-3-penten-2-one, 4-methyl-2-pentanone, 4-methyl-2-pentanol, acetone, 1,3-butendiol, etanol, 2-propanol, 3-hydroxybutyrate, and 2-oxobutyrate, substrate specificity, overview. Product identification by GC-MS analysis
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(4R)-ipsdienol + NAD(P)+
ipsdienone + NAD(P)H + H+
the enzyme is involved in pheromon production
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?
(R)-ipsdienol + NADP+
ipsdienone + NADPH + H+
stereospecific reaction
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r
racemic ipsenol + NADP+
ipsenone + NADPH + H+
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r
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NAD+
the enzyme shows similar specific activities with NAD+ and NADP+
NADP+
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NADP+
the enzyme shows similar specific activities with NAD+ and NADP+
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additional information
1,3-butanediol, nerol, geraniol, citral, (S)-ipsdienol do not act as inhibitors
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additional information
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1,3-butanediol, nerol, geraniol, citral, (S)-ipsdienol do not act as inhibitors
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0.58
(4R)-ipsdienol
pH 7.8, 25°C
0.527
(R)-ipsdienol
pH 7.5, 25°C, recombinant enzyme
0.002
ipsdienone
pH 7.5, 25°C, recombinant enzyme
0.003
ipsenone
pH 7.5, 25°C, recombinant enzyme
0.000008
NADP+
pH 7.5, 25°C, recombinant enzyme, with (R)-ipsdienol
0.009
racemic ipsenol
pH 7.5, 25°C, recombinant enzyme
additional information
additional information
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additional information
additional information
kinetics, recombinant enzyme
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additional information
additional information
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kinetics, recombinant enzyme
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0.03
(-)-menthone
pH 7.5, 25°C, recombinant enzyme
33.6
1-propanol
pH 7.5, 25°C, recombinant enzyme
9.9
2-propanol
pH 7.5, 25°C, recombinant enzyme
0.015
ipsdienone
pH 7.5, 25°C, recombinant enzyme
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0.05
partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsdienone
0.092
partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsenone
0.196
partially purified recombinant enzyme, pH 7.5, 25°C, substrate racemic ipsenol
0.52 - 0.525
partially purified recombinant enzyme, pH 7.5, 25°C, Tris-HCl or phosphate buffer, substrate (R)-ipsdienol
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25
assay at
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5.8
calculated from sequence
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UniProt
brenda
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transcript levels are highest in pheromone producing tissue: the anterior midgut of fed males. The protein is detected only in male midguts
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additional information
IDOLDH expression pattern, overview
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additional information
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IDOLDH expression pattern, overview
brenda
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evolution
the enzyme belongs to the short-chain oxidoreductase ipsdienol dehydrogenases
metabolism
the enzyme is involved in pheromon production
physiological function
Ips spp. bark beetles use ipsdienol, ipsenol, ipsdienone and ipsenone as aggregation pheromone components and pheromone precursors. For Ips pini, the short-chain oxidoreductase ipsdienol dehydrogenase (IDOLDH) converts (R)-ipsdienol to ipsdienone, and thus likely plays a role in determining pheromone composition. Different IDOLDH orthologues and or activity levels contribute to differences in Ips spp. pheromone composition
additional information
the enzyme contains the typical Rossmann-fold motif for nucleotide binding and the catalytic tetrad that are highly conserved
additional information
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the enzyme contains the typical Rossmann-fold motif for nucleotide binding and the catalytic tetrad that are highly conserved
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IDLDH_IPSPI
253
0
27514
Swiss-Prot
other Location (Reliability: 2 )
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117000
recombinant enzyme, gel filtration
28000
calculated from sequence
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homotetramer
4 * 27500, recombinant enzyme, SDS-PAGE, functional enzyme form
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recombinant enzyme about 2.5fold from Sf9 cells by ammonium sulfate fractionation
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gene IDOLDH, recombinant expression in Spodoptera frugiperda Sf9 cells via baculovirus transfection method
the recombinant enzyme is produced in Sf9 cells using a baculovirus expression system
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Figueroa-Teran, R.; Welch, W.H.; Blomquist, G.J.; Tittiger, C.
Ipsdienol dehydrogenase (IDOLDH): a novel oxidoreductase important for Ips pini pheromone production
Insect Biochem. Mol. Biol.
42
81-90
2012
Ips pini (G5DGA8), Ips pini
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Figueroa-Teran, R.; Pak, H.; Blomquist, G.J.; Tittiger, C.
High substrate specificity of ipsdienol dehydrogenase (IDOLDH), a short-chain dehydrogenase from Ips pini bark beetles
J. Biochem.
160
141-151
2016
Ips pini (G5DGA8), Ips pini
brenda
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