3.1.21.B3: mismatch-specific endonuclease

This is an abbreviated version!
For detailed information about mismatch-specific endonuclease, go to the full flat file.

Reaction

endonucleolytic cleavage of both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. The enzyme cleaves G/T, G/G, T/T, T/C and A/G mismatches, with a preference for G/T, G/G and T/T. It cleaves the third phosphodiester bond on the 5' side of the mismatched base in both strands, to produce a cohesive end with a five-nucleotide long 5'-protrusion, leaving 5'-phosphate and 3'-hydroxyl termini. =

Synonyms

endonuclease NucS, TK1898, TkEndoMS

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

                3.1.21.B3 mismatch-specific endonuclease

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Results	in table
1	AA Sequence
2	Application
2	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
1	General Stability
2	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
6	Organism
1	pH Range
2	Protein Variants
2	Purification (Commentary)
1	Reaction
4	Reference
10	Substrates and Products (Substrate)
2	Subunits
8	Synonyms
1	Temperature Optimum [°C]
1	Temperature Stability [°C]